PMID- 15297496
OWN - NLM
STAT- MEDLINE
DCOM- 20040930
LR  - 20181113
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 42
IP  - 8
DP  - 2004 Aug
TI  - Use of 16S ribosomal DNA PCR and denaturing gradient gel electrophoresis for 
      analysis of the microfloras of healing and nonhealing chronic venous leg ulcers.
PG  - 3549-57
AB  - The bacterial microfloras of 8 healing and 10 nonhealing chronic venous leg 
      ulcers were compared by using a combination of cultural analysis and denaturing 
      gradient gel electrophoresis (DGGE) of PCR-amplified 16S rRNA gene products. 
      Cultural analysis of the microflora revealed that the majority of both wound 
      types carried the aerobes Staphylococcus and Pseudomonas spp. (89 and 80%, 
      respectively). Sequencing of 16S ribosomal DNAs selected on the basis of DGGE 
      profiling allowed the identification of strains not detected by cultural means. 
      Of considerable interest was the finding that more than 40% of the sequences 
      represented organisms not cultured from the wound from which they were amplified. 
      DGGE profiles also revealed that all of the wounds possessed one apparently 
      common band, identified by sequencing as Pseudomonas sp. The intensity of this 
      PCR signal suggested that the bacterial load of nonhealing wounds was much higher 
      for pseudomonads compared to healing wounds and that it may have been 
      significantly underestimated by cultural analysis. Hence, the present study shows 
      that DGGE could give valuable additional information about chronic wound 
      microflora that is not apparent from cultural analysis alone.
FAU - Davies, Charlotte E
AU  - Davies CE
AD  - Wound Biology Group, Department of Oral Surgery, Medicine, and Pathology, 
      University of Wales College of Medicine, Heath Park, Cardiff CF14 4XY, United 
      Kingdom.
FAU - Hill, Katja E
AU  - Hill KE
FAU - Wilson, Melanie J
AU  - Wilson MJ
FAU - Stephens, Phil
AU  - Stephens P
FAU - Hill, C Michael
AU  - Hill CM
FAU - Harding, Keith G
AU  - Harding KG
FAU - Thomas, David W
AU  - Thomas DW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Base Sequence
MH  - Biopsy
MH  - Chronic Disease
MH  - DNA, Ribosomal/*genetics
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Humans
MH  - Leg Ulcer/*microbiology/pathology
MH  - Polymerase Chain Reaction/*methods
MH  - Pseudomonas/isolation & purification
MH  - RNA, Ribosomal, 16S/*genetics
MH  - Staphylococcus/isolation & purification
MH  - *Wound Healing
PMC - PMC497624
EDAT- 2004/08/07 05:00
MHDA- 2004/10/02 05:00
PMCR- 2004/08/01
CRDT- 2004/08/07 05:00
PHST- 2004/08/07 05:00 [pubmed]
PHST- 2004/10/02 05:00 [medline]
PHST- 2004/08/07 05:00 [entrez]
PHST- 2004/08/01 00:00 [pmc-release]
AID - 42/8/3549 [pii]
AID - 1074-03 [pii]
AID - 10.1128/JCM.42.8.3549-3557.2004 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2004 Aug;42(8):3549-57. doi: 10.1128/JCM.42.8.3549-3557.2004.