PMID- 15298576
OWN - NLM
STAT- MEDLINE
DCOM- 20041220
LR  - 20061115
IS  - 0954-7894 (Print)
IS  - 0954-7894 (Linking)
VI  - 34
IP  - 8
DP  - 2004 Aug
TI  - Enhanced airway inflammation and decreased subepithelial fibrosis in interleukin 
      6-deficient mice following chronic exposure to aerosolized antigen.
PG  - 1321-8
AB  - BACKGROUND: Airway inflammation and remodelling are characteristic features of 
      chronic asthma. OBJECTIVE: To elucidate the role of interleukin (IL)-6 in airway 
      responses to chronic antigen exposure. METHODS: We compared airway inflammation, 
      subepithelial collagen deposition, cytokine mRNA expression, and airway 
      responsiveness between IL-6-deficient and wild-type (WT) mice following 
      sensitization and repeated exposure to ovalbumin (OVA) three times a week for 8 
      weeks. RESULTS: The repeated exposure to OVA induced infiltration of eosinophils, 
      neutrophils, and lymphocytes into the airway, and caused thickening of the 
      basement membrane and subepithelial fibrosis. IL-6-deficient mice exhibited more 
      pronounced infiltration of these cells, a thinner basement membrane, and 
      decreased subepithelial fibrosis, compared with WT mice. The repeated OVA 
      exposure increased expression of IL-4, IL-13, eotaxin, monocyte chemoattractant 
      protein-1 (MCP-1), and transforming growth factor-beta1 mRNA in WT mice. Among 
      these factors, expression of IL-13 and MCP-1 mRNA was further enhanced in 
      IL-6-deficient mice, compared with WT mice. However, both WT and IL-6-deficient 
      mice exhibited similar levels of airway responsiveness to increasing doses of 
      methacholine, even after repeated exposure to OVA. CONCLUSION: These results 
      suggest that IL-6 has dual roles in the chronic phase of asthma: down-regulation 
      of inflammatory cell infiltration and enhancement of airway remodelling.
FAU - Qiu, Z
AU  - Qiu Z
AD  - Respiratory Medicine, Cellular Transplantation Biology, Kanazawa Graduate 
      University School of Medicine, Kanazawa, Japan.
FAU - Fujimura, M
AU  - Fujimura M
FAU - Kurashima, K
AU  - Kurashima K
FAU - Nakao, S
AU  - Nakao S
FAU - Mukaida, N
AU  - Mukaida N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Exp Allergy
JT  - Clinical and experimental allergy : journal of the British Society for Allergy 
      and Clinical Immunology
JID - 8906443
RN  - 0 (Aerosols)
RN  - 0 (Allergens)
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
RN  - 0 (Growth Substances)
RN  - 0 (Interleukin-6)
RN  - 9006-59-1 (Ovalbumin)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Aerosols
MH  - Allergens/*administration & dosage
MH  - Animals
MH  - Asthma/*immunology/pathology
MH  - Bronchoalveolar Lavage Fluid/cytology
MH  - Cell Count
MH  - Chemokines/analysis
MH  - Chronic Disease
MH  - Collagen/analysis
MH  - Cytokines/analysis
MH  - Female
MH  - Fibrosis
MH  - Growth Substances/analysis
MH  - Interleukin-6/genetics/*immunology
MH  - Lung/*immunology/pathology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Knockout
MH  - Models, Animal
MH  - Ovalbumin
EDAT- 2004/08/10 05:00
MHDA- 2004/12/21 09:00
CRDT- 2004/08/10 05:00
PHST- 2004/08/10 05:00 [pubmed]
PHST- 2004/12/21 09:00 [medline]
PHST- 2004/08/10 05:00 [entrez]
AID - CEA2013 [pii]
AID - 10.1111/j.1365-2222.2004.02013.x [doi]
PST - ppublish
SO  - Clin Exp Allergy. 2004 Aug;34(8):1321-8. doi: 10.1111/j.1365-2222.2004.02013.x.