PMID- 15298772
OWN - NLM
STAT- MEDLINE
DCOM- 20041007
LR  - 20131121
IS  - 1096-620X (Print)
IS  - 1096-620X (Linking)
VI  - 7
IP  - 2
DP  - 2004 Summer
TI  - Methionine-induced hyperhomocysteinemia promotes superoxide anion generation and 
      NFkappaB activation in peritoneal macrophages of C57BL/6 mice.
PG  - 229-34
AB  - It is well documented that hyperhomocysteinemia (HHcy) is an independent risk 
      factor for atherosclerosis. This study was designed to investigate whether some 
      of the atherosclerotic effects ascribed to HHcy are mediated by oxidative stress 
      and nuclear factor kappa B (NFkappaB) activation in peritoneal macrophages of 
      C57BL/6 mice fed a high (2%) methionine/low (1 mg/kg) folate diet for 12 weeks. 
      Plasma homocysteine concentrations in mice fed methionine averaged 49 mol/L after 
      12 weeks of feeding, five times higher than that of controls. HHcy induced by 
      methionine feeding significantly elevated oxidative stress, as measured by 
      superoxide anion radical level (P <.05) in peritoneal macrophages. Furthermore, 
      NFkappaB binding activities of peritoneal macrophages were higher in the 
      methionine group than in the control group. These results suggest that HHcy 
      induced by methionine may intensify disturbances in peroxidation and inflammatory 
      mediator activation in peritoneal macrophages, and is a possible mechanism of its 
      atherogenic effects.
FAU - Song, Young-Sun
AU  - Song YS
AD  - School of Food and Life Science and Food Science Institute, Inje University, 
      Obang-dong, Kimhae, Kyung-Nam, Korea. fdsnsong@inje.ac.kr
FAU - Rosenfeld, Michael E
AU  - Rosenfeld ME
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Med Food
JT  - Journal of medicinal food
JID - 9812512
RN  - 0 (Anions)
RN  - 0 (NF-kappa B)
RN  - 11062-77-4 (Superoxides)
RN  - AE28F7PNPL (Methionine)
SB  - IM
MH  - Animals
MH  - Anions
MH  - Cells, Cultured
MH  - Diet
MH  - Hyperhomocysteinemia/chemically induced/*metabolism
MH  - Kinetics
MH  - Macrophages, Peritoneal/*metabolism
MH  - Methionine/*administration & dosage
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - NF-kappa B/*metabolism
MH  - Oxidative Stress
MH  - Superoxides/*metabolism
EDAT- 2004/08/10 05:00
MHDA- 2004/10/08 09:00
CRDT- 2004/08/10 05:00
PHST- 2004/08/10 05:00 [pubmed]
PHST- 2004/10/08 09:00 [medline]
PHST- 2004/08/10 05:00 [entrez]
AID - 10.1089/1096620041224021 [doi]
PST - ppublish
SO  - J Med Food. 2004 Summer;7(2):229-34. doi: 10.1089/1096620041224021.