PMID- 15298980
OWN - NLM
STAT- MEDLINE
DCOM- 20050323
LR  - 20061115
IS  - 0268-1161 (Print)
IS  - 0268-1161 (Linking)
VI  - 19
IP  - 11
DP  - 2004 Nov
TI  - Independent regulation of prostaglandins and monocyte chemoattractant protein-1 
      by interleukin-1beta and hCG in human endometrial cells.
PG  - 2465-73
AB  - BACKGROUND: Inflammatory mediators such as prostaglandins (PG), chemokines, 
      cytokines and their interactions regulate reproductive functions. The 
      relationship between PG and monocyte chemoattractant protein-1 (MCP-1) has not 
      been elucidated in human endometrium. The presence of hCG receptors in the human 
      endometrium suggests that this embryonic signal may exert a local function in 
      this tissue. Our objectives were to investigate the possible association between 
      PG and MCP-1 and to examine the role of hCG in interleukin-1beta 
      (IL-1beta)-regulated PG and MCP-1 production in human endometrium. METHODS: 
      Primary cell cultures isolated from endometrial biopsies were used as an in vitro 
      model. PG and MCP-1 levels were measured in the culture medium. RESULTS: IL-1beta 
      stimulates the production of both PG and MCP-1. Neither COX inhibitors nor direct 
      addition of PG affects MCP-1 production. By contrast, MCP-1 is able to induce 
      PGE2 and PGF2alpha in a concentration-dependent manner but it does not appear to 
      contribute to the increase in PG accumulation following IL-1beta stimulation. hCG 
      inhibits IL-1beta-induced PG level. However, hCG has no effect on either basal or 
      IL-1beta-mediated MCP-1 level. CONCLUSIONS: PG are not involved in the regulation 
      of MCP-1 production in endometrial cells. hCG appears to play a local function in 
      the endometrium.
FAU - Kang, Jihong
AU  - Kang J
AD  - Unite de Recherche en Ontogenie et Reproduction et Centre de Recherche en 
      Rhumatologie et Immunologie, Centre de Recherche du CHUL, 2705 Boul. Laurier, 
      Ste-Foy, Quebec, G1V 4G2, Canada.
FAU - Akoum, Ali
AU  - Akoum A
FAU - Chapdelaine, Pierre
AU  - Chapdelaine P
FAU - Laberge, Philippe
AU  - Laberge P
FAU - Poubelle, Patrice E
AU  - Poubelle PE
FAU - Fortier, Michel A
AU  - Fortier MA
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20040806
PL  - England
TA  - Hum Reprod
JT  - Human reproduction (Oxford, England)
JID - 8701199
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chorionic Gonadotropin)
RN  - 0 (Culture Media)
RN  - 0 (Cyclooxygenase 2 Inhibitors)
RN  - 0 (Cyclooxygenase Inhibitors)
RN  - 0 (Interleukin-1)
RN  - 0 (Membrane Proteins)
RN  - 0 (Prostaglandins)
RN  - EC 1.14.99.1 (Cyclooxygenase 1)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
RN  - EC 1.14.99.1 (PTGS1 protein, human)
RN  - EC 1.14.99.1 (PTGS2 protein, human)
RN  - EC 1.14.99.1 (Prostaglandin-Endoperoxide Synthases)
SB  - IM
MH  - Adult
MH  - Cells, Cultured
MH  - Chemokine CCL2/drug effects/*metabolism
MH  - Chorionic Gonadotropin/metabolism/*pharmacology
MH  - Culture Media
MH  - Cyclooxygenase 1
MH  - Cyclooxygenase 2
MH  - Cyclooxygenase 2 Inhibitors
MH  - Cyclooxygenase Inhibitors/pharmacology
MH  - Endometrium/*cytology/drug effects/physiology
MH  - Female
MH  - Humans
MH  - Interleukin-1/metabolism/*pharmacology
MH  - Membrane Proteins
MH  - Middle Aged
MH  - Prostaglandin-Endoperoxide Synthases/drug effects/metabolism
MH  - Prostaglandins/*metabolism
MH  - Stromal Cells/drug effects/metabolism
MH  - Time Factors
EDAT- 2004/08/10 05:00
MHDA- 2005/03/24 09:00
CRDT- 2004/08/10 05:00
PHST- 2004/08/10 05:00 [pubmed]
PHST- 2005/03/24 09:00 [medline]
PHST- 2004/08/10 05:00 [entrez]
AID - deh458 [pii]
AID - 10.1093/humrep/deh458 [doi]
PST - ppublish
SO  - Hum Reprod. 2004 Nov;19(11):2465-73. doi: 10.1093/humrep/deh458. Epub 2004 Aug 6.