PMID- 15299309
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20050215
LR  - 20070724
IS  - 0907-4449 (Print)
IS  - 0907-4449 (Linking)
VI  - 51
IP  - Pt 3
DP  - 1995 May 1
TI  - Preliminary crystallographic analysis of an extremely thermostable glutamate 
      dehydrogenase from the archaeon Pyrococcus woesei.
PG  - 395-8
AB  - The extremely heat-stable glutamate dehydrogenase (GluDH) from Pyrococcus woesei 
      was crystallized by the hanging-drop vapour-diffusion method. Crystals suitable 
      for X-ray crystallographic investigations were obtained using polyethylene glycol 
      (PEG) 4000 and ammonium acetate as precipitating agents. The crystals obtained 
      diffract to a resolution of 2.8 A, have a prismatic shape and grow up to 0.5 mm 
      in their maximal dimension. They belong to the triclinic system (space group P1; 
      a = 90.9, b = 92.8, c = 107.1 A, alpha = 69.1, beta = 80.7, Vgamma = 65.0 degrees 
      ) with a unit-cell volume of 765052 A(3) which accommodates one GluDH hexamer of 
      276 kDa. The averaged density of the crystal determined by Ficoll-gradient 
      centrifugation is 1.15 g cm(-3), which corresponds to a molecular mass of 255 kDa 
      in the unit cell. A native data set has been collected on an MAR image-plate 
      system using Cu Kalpha radiation. The completeness of the data set in the range 
      316-3 A is 74%, and contains 64% of the data in the outer shell (3.4-2.8 A), with 
      an average R(merge) value of 9%. Calculation of self-rotation functions revealed 
      the 32 symmetry of the hexamer; 3 non-crystallographic twofold axes were found at 
      a distance of 120 degrees in a plane perpendicular to the non-crystallographic 
      threefold axis.
FAU - Knapp, S
AU  - Knapp S
AD  - Karolinska Institute, NOVUM, Center for Structural Biochemsitry, Huddinge, 
      Sweden.
FAU - Karshikoff, A
AU  - Karshikoff A
FAU - Waldkotter, K
AU  - Waldkotter K
FAU - Rudiger, A
AU  - Rudiger A
FAU - Antranikian, G
AU  - Antranikian G
FAU - Ladenstein, R
AU  - Ladenstein R
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Acta Crystallogr D Biol Crystallogr
JT  - Acta crystallographica. Section D, Biological crystallography
JID - 9305878
EDAT- 1995/05/01 00:00
MHDA- 1995/05/01 00:01
CRDT- 1995/05/01 00:00
PHST- 1995/05/01 00:00 [pubmed]
PHST- 1995/05/01 00:01 [medline]
PHST- 1995/05/01 00:00 [entrez]
AID - S0907444994011224 [pii]
AID - 10.1107/S0907444994011224 [doi]
PST - ppublish
SO  - Acta Crystallogr D Biol Crystallogr. 1995 May 1;51(Pt 3):395-8. doi: 
      10.1107/S0907444994011224.