PMID- 15300511
OWN - NLM
STAT- MEDLINE
DCOM- 20041210
LR  - 20141120
IS  - 0214-6282 (Print)
IS  - 0214-6282 (Linking)
VI  - 48
IP  - 4
DP  - 2004 Jun
TI  - Terminal differentiation of palatal medial edge epithelial cells in vitro is not 
      necessarily dependent on palatal shelf contact and midline epithelial seam 
      formation.
PG  - 307-17
AB  - During fusion of the mammalian secondary palate, it has been suggested that 
      palatal medial edge epithelial (MEE) cells disappear by means of apoptosis, 
      epithelial-mesenchymal transformation (EMT) and epithelial cell migration. 
      However, it is widely believed that MEE cells never differentiate unless palatal 
      shelves make contact and the midline epithelial seam is formed. In order to 
      clarify the potential of MEE cells to differentiate, we cultured single 
      (unpaired) palatal shelves of ICR mouse fetuses by using suspension and static 
      culture methods with two kinds of gas-mixtures. We thereby found that MEE cells 
      can disappear throughout the medial edge even without contact and adhesion to the 
      opposing MEE in suspension culture with 95% O2/5% CO2. Careful examination of MEE 
      cell behavior in the culture revealed that apoptosis, EMT, and epithelial cell 
      migration all occurred at various stages of MEE cell disappearance, including the 
      transient formation and disappearance of epithelial triangles and islets. In 
      contrast, MEE cells showed poor differentiation in static culture in a CO2 
      incubator. Furthermore, mouse and human amniotic fluids were found to prevent MEE 
      cell differentiation in the cultured single palatal shelf, although paired 
      palatal shelves fused successfully even in the presence of amniotic fluid. We 
      therefore conclude that terminal differentiation of MEE cells is not necessarily 
      dependent on palatal shelf contact and midline epithelial seam formation, but 
      such MEE cell differentiation appears to be prevented in utero by amniotic fluid 
      unless palatal shelves make close contact and the midline epithelial seam is 
      formed.
FAU - Takigawa, Toshiya
AU  - Takigawa T
AD  - Department of Anatomy and Developmental Biology, Kyoto University Graduate School 
      of Medicine, Kyoto, Japan. takigawa@cac.med.kyoto-u.ac.jp
FAU - Shiota, Kohei
AU  - Shiota K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Spain
TA  - Int J Dev Biol
JT  - The International journal of developmental biology
JID - 8917470
SB  - IM
MH  - Amniotic Fluid/physiology
MH  - Animals
MH  - Apoptosis
MH  - Cell Differentiation
MH  - Epithelial Cells/cytology
MH  - Female
MH  - Humans
MH  - Mesoderm/cytology
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Microscopy, Electron, Scanning
MH  - Palate/cytology/*embryology
MH  - Pregnancy
MH  - Tissue Culture Techniques
EDAT- 2004/08/10 05:00
MHDA- 2004/12/16 09:00
CRDT- 2004/08/10 05:00
PHST- 2004/08/10 05:00 [pubmed]
PHST- 2004/12/16 09:00 [medline]
PHST- 2004/08/10 05:00 [entrez]
AID - 041840tt [pii]
AID - 10.1387/ijdb.041840tt [doi]
PST - ppublish
SO  - Int J Dev Biol. 2004 Jun;48(4):307-17. doi: 10.1387/ijdb.041840tt.