PMID- 15300644
OWN - NLM
STAT- MEDLINE
DCOM- 20090303
LR  - 20040809
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 21
IP  - 4
DP  - 2004 Aug
TI  - [Detection of abnormal numbers of chromosome 8 with interphase fluorescence in 
      situ hybridization in hematologic malignancies].
PG  - 395-7
AB  - OBJECTIVE: To explore the value of interphase fluorescence in situ hybridization 
      (FISH) in the detection of abnormal numbers of chromosome 8 in patients with 
      hematologic malignancies. METHODS: Conventional cytogenetics(CC) and interphase 
      FISH using chromosome 8 centromere specific probe were simultaneously carried out 
      to detect the abnormal numbers of chromosome 8 in eight acute myeloid leukemia 
      cases with CC unveiled abnormal numbers of chromosome 8, ten chronic myeloid 
      leukemia cases in accelerated phase or blast crisis, and three normal 
      individuals. RESULTS: Nine cases that displayed trisomy 8 by means of CC were 
      confirmed by FISH. Among them, Case 5 only displayed diploidy 8, trisomy 8 and 
      tetrasomy 8 by CC, at the same time, FISH confirmed the presence of trisomy 8 and 
      tetrasomy 8 and also revealed a low percentage of a pentasomy 8 clone. Case 3 and 
      Case 17 had each only one cell with trisomy 8 by means of CC, and this could not 
      determine whether they had the trisomy 8 clone, yet FISH confirmed the existence 
      of trisomy 8 clone. Case 9 did not display trisomy 8 by CC, but FISH revealed the 
      existence of trisomy 8 clone. In the other cases, the percentages of trisomy 8 
      cells determined by FISH were close to or significantly lower than those by CC, 
      but for Case 16 where the percentage of trisomy 8 cells by FISH was significantly 
      higher than that by CC. CONCLUSION: Interphase FISH is a useful method for the 
      detection of abnormal numbers of chromosome 8, especially in the patients with 
      normal or unsure karyotype or with less and bad metaphases. It is an important 
      complement to CC.
FAU - Wang, Hui-ping
AU  - Wang HP
AD  - Department of Hematology, Second Hospital of Shanxi Medical University, Taiyuan, 
      Shanxi, 030001 PR China. tywhp9618@msn.com
FAU - Li, Guo-xia
AU  - Li GX
FAU - Qiao, Zhen-hua
AU  - Qiao ZH
FAU - Wang, Hong-wei
AU  - Wang HW
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
SB  - IM
MH  - Acute Disease
MH  - Adolescent
MH  - Adult
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human, Pair 8/*genetics
MH  - Female
MH  - Hematologic Neoplasms/diagnosis/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/diagnosis/genetics
MH  - Leukemia, Myeloid/diagnosis/genetics
MH  - Male
MH  - Middle Aged
MH  - Reproducibility of Results
EDAT- 2004/08/10 05:00
MHDA- 2009/03/04 09:00
CRDT- 2004/08/10 05:00
PHST- 2004/08/10 05:00 [pubmed]
PHST- 2009/03/04 09:00 [medline]
PHST- 2004/08/10 05:00 [entrez]
AID - 940621099 [pii]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2004 Aug;21(4):395-7.