PMID- 15300749
OWN - NLM
STAT- MEDLINE
DCOM- 20050414
LR  - 20220311
IS  - 0197-3851 (Print)
IS  - 0197-3851 (Linking)
VI  - 24
IP  - 7
DP  - 2004 Jul
TI  - Efficacy and clinical outcome of preimplantation genetic diagnosis using FISH for 
      couples of reciprocal and Robertsonian translocations: the Korean experience.
PG  - 556-61
AB  - OBJECTIVE: To evaluate the efficacy and clinical outcome of preimplantation 
      genetic diagnosis (PGD) using fluorescence in situ hybridization (FISH) for 
      couples with chromosomal translocations. METHODS: PGD using FISH was performed in 
      59 cycles of 43 couples with reciprocal translocations, and 11 cycles of 6 
      couples with Robertsonian translocations. The diagnostic and clinical data were 
      reviewed in a series of 70 treatment cycles of 49 couples from January 2001 to 
      June 2002 at Samsung Cheil Hospital, Korea. RESULTS: A total of 1408 oocytes were 
      retrieved, and 938 (81.7%) out of 1148 matured oocytes were fertilized by 
      intracytoplasmic sperm injection (ICSI). Single blastomere biopsy and FISH 
      analysis were successfully carried out in 99.3% (890/896) and 94.4% (840/890), 
      respectively. Among 193 normal or balanced embryos, 169 embryos were transferred 
      in 64 cycles (91.4% per started cycle). Twenty clinical pregnancies including two 
      ectopic pregnancies and three spontaneous miscarriages (28.6% per started cycle, 
      31.3% per transfer cycle, 40.8% per couple) were established. Of the three 
      spontaneous miscarriages, one was karyotyped as normal, one had an unbalanced 
      arrangement and one was tetraploid. One case of preterm twin delivery occurred 
      and 16 healthy babies were delivered in 12 single and 2 twin pregnancies. 
      CONCLUSION: The clinical outcome was successful in 28.6% (14/49) of the treated 
      couples with translocations after PGD. The spontaneous abortion rate was 
      significantly reduced from 95.8% (69/72) to 16.7% (3/18) in these couples.
CI  - Copyright 2004 John Wiley and Sons, Ltd.
FAU - Kyu Lim, Chun
AU  - Kyu Lim C
AD  - Laboratory of Reproductive Biology and Infertility, Samsung Cheil Hospital and 
      Women's Healthcare Center, Sungkyunkwan University School of Medicine, Seoul, 
      Korea.
FAU - Hyun Jun, Jin
AU  - Hyun Jun J
FAU - Mi Min, Dong
AU  - Mi Min D
FAU - Lee, Hyoung-Song
AU  - Lee HS
FAU - Young Kim, Jin
AU  - Young Kim J
FAU - Koong, Mi Kyoung
AU  - Koong MK
FAU - Kang, Inn Soo
AU  - Kang IS
LA  - eng
PT  - Journal Article
PL  - England
TA  - Prenat Diagn
JT  - Prenatal diagnosis
JID - 8106540
SB  - IM
CIN - Prenat Diagn. 2004 Jul;24(7):552. PMID: 15300747
CIN - Prenat Diagn. 2004 Jul;24(7):553-5. PMID: 15300748
MH  - Abortion, Spontaneous/*prevention & control
MH  - Biopsy
MH  - Blastomeres
MH  - Chromosome Disorders/*diagnosis/embryology
MH  - Embryo Culture Techniques
MH  - Evaluation Studies as Topic
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence/methods
MH  - Korea
MH  - Lymphocytes/ultrastructure
MH  - Male
MH  - Ovulation Induction
MH  - Pregnancy
MH  - Pregnancy Outcome
MH  - *Preimplantation Diagnosis/methods
MH  - Translocation, Genetic/*genetics
EDAT- 2004/08/10 05:00
MHDA- 2005/04/15 09:00
CRDT- 2004/08/10 05:00
PHST- 2004/08/10 05:00 [pubmed]
PHST- 2005/04/15 09:00 [medline]
PHST- 2004/08/10 05:00 [entrez]
AID - 10.1002/pd.923 [doi]
PST - ppublish
SO  - Prenat Diagn. 2004 Jul;24(7):556-61. doi: 10.1002/pd.923.