PMID- 15303729
OWN - NLM
STAT- MEDLINE
DCOM- 20041018
LR  - 20240109
IS  - 0273-1223 (Print)
IS  - 0273-1223 (Linking)
VI  - 49
IP  - 11-12
DP  - 2004
TI  - High nitrification rate at low pH in a fluidized bed reactor with chalk as the 
      biofilm carrier.
PG  - 99-105
AB  - A typical steady state bulk pH of about 5 was established in a nitrifying 
      fluidized bed with chalk as the only buffer agent. In spite of the low pH, high 
      rate nitrification was observed with the nitrification kinetic parameters in the 
      chalk reactor similar to those of biological reactors operating at pH>7. Various 
      methods were used to determine the reasons for high rate nitrification at such 
      low pH including (i) determination of bacterial species, (ii) microsensor 
      measurements in the biofilm, and (iii) comparison of nitrification performance at 
      low pH with a non-chalk fluidized bed reactor. Fluorescence in situ hybridization 
      (FISH) using existing 16S rRNA-targeted oligonucleotide probes showed common 
      nitrifying bacteria in the low pH chalk reactor. The prevalent nitrifying 
      bacteria were identified in the Nitrosomonas oligotropha, Nitrosomonas 
      europeae/eutropha, Nitrosospira and Nitrospira related groups, all well known 
      nitrifiers. Microelectrode measurements showed that the pH in the biofilm was low 
      and similar to that of the bulk pH. Finally, reactor performance using a 
      non-chalk biofilm carrier (sintered glass) with the same bacterial inoculum also 
      showed high rate nitrification below pH 5. The results suggest that inhibition of 
      nitrification at low pH is highly overestimated.
FAU - Tarre, S
AU  - Tarre S
AD  - Faculty of Civil and Environmental Engineering, Technion, Haifa, Israel. 
      shelly@tx.technion.ac.il
FAU - Beliavski, M
AU  - Beliavski M
FAU - Denekamp, N
AU  - Denekamp N
FAU - Gieseke, A
AU  - Gieseke A
FAU - de Beer, D
AU  - de Beer D
FAU - Green, M
AU  - Green M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Water Sci Technol
JT  - Water science and technology : a journal of the International Association on 
      Water Pollution Research
JID - 9879497
RN  - 0 (DNA, Bacterial)
RN  - H0G9379FGK (Calcium Carbonate)
RN  - N762921K75 (Nitrogen)
SB  - IM
MH  - *Biofilms
MH  - *Bioreactors
MH  - Calcium Carbonate/chemistry
MH  - DNA, Bacterial/analysis
MH  - Hydrogen-Ion Concentration
MH  - In Situ Hybridization, Fluorescence
MH  - Nitrogen/*isolation & purification
MH  - Nitrosomonas/growth & development/physiology
MH  - Waste Disposal, Fluid/*methods
EDAT- 2004/08/12 05:00
MHDA- 2004/10/19 09:00
CRDT- 2004/08/12 05:00
PHST- 2004/08/12 05:00 [pubmed]
PHST- 2004/10/19 09:00 [medline]
PHST- 2004/08/12 05:00 [entrez]
PST - ppublish
SO  - Water Sci Technol. 2004;49(11-12):99-105.