PMID- 15303763
OWN - NLM
STAT- MEDLINE
DCOM- 20041018
LR  - 20131121
IS  - 0273-1223 (Print)
IS  - 0273-1223 (Linking)
VI  - 49
IP  - 11-12
DP  - 2004
TI  - Transition of bacterial spatial organization in a biofilm monitored by FISH and 
      subsequent image analysis.
PG  - 365-70
AB  - The dynamic transition of bacterial community structure in a biofilm was 
      monitored by the fluorescence in situ hybridization (FISH) technique and 
      subsequent image analysis. Heterotrophic bacteria that had occupied the outer 
      layer were gradually decreased whereas ammonia-oxidizing bacteria (AOB) gradually 
      increased their growth activity and extended their existence area to the outer 
      layer of the biofilm through the gradual reduction of the C/N ratio. The spatial 
      organization of AOB in the biofilm dynamically changed responding to the 
      environmental conditions such as pH fluctuation and lack of dissolved oxygen (DO) 
      and had great influence on the nitrification activity. The accumulation of 
      nitrite was observed at lower DO concentration, which might be due to the 
      property that nitrite-oxidizing bacteria (NOB) possess of higher Km values for 
      oxygen than AOB.
FAU - Aoi, Y
AU  - Aoi Y
AD  - Department of Chemical Engineering, Waseda University, Ohkubo, Shinjuku-ku, 
      Tokyo, Japan. yoshiteruaoi@toki.waseda.jp
FAU - Tsuneda, S
AU  - Tsuneda S
FAU - Hirata, A
AU  - Hirata A
LA  - eng
PT  - Journal Article
PL  - England
TA  - Water Sci Technol
JT  - Water science and technology : a journal of the International Association on 
      Water Pollution Research
JID - 9879497
RN  - 0 (DNA, Bacterial)
RN  - 0 (Nitrites)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Bacteria/growth & development
MH  - Biofilms/*growth & development
MH  - DNA, Bacterial/analysis
MH  - Environmental Monitoring/methods
MH  - Hydrogen-Ion Concentration
MH  - *In Situ Hybridization, Fluorescence
MH  - Nitrites/analysis
MH  - Oxygen/analysis
EDAT- 2004/08/12 05:00
MHDA- 2004/10/19 09:00
CRDT- 2004/08/12 05:00
PHST- 2004/08/12 05:00 [pubmed]
PHST- 2004/10/19 09:00 [medline]
PHST- 2004/08/12 05:00 [entrez]
PST - ppublish
SO  - Water Sci Technol. 2004;49(11-12):365-70.