PMID- 15304010
OWN - NLM
STAT- MEDLINE
DCOM- 20050307
LR  - 20221207
IS  - 0001-2815 (Print)
IS  - 0001-2815 (Linking)
VI  - 64
IP  - 3
DP  - 2004 Sep
TI  - Molecular typing of human leukocyte antigen and related polymorphisms following 
      whole genome amplification.
PG  - 286-92
AB  - Reliable, high-resolution genotyping of human leukocyte antigen (HLA) 
      polymorphisms is often compromised by DNA samples of suboptimal quality or 
      limited quantity. We tested the feasibility of molecular typing for variants at 
      HLA and neighboring loci using whole genome amplification (WGA) strategy 
      facilitated by the Phi29 DNA polymerase. With little (5-100 ng) starting genomic 
      DNA of varying quality and source materials, WGA was deemed successful in 167 of 
      169 DNA from 47 cell lines, 100 European Americans, and 22 native Africans. The 
      Phi29-processed DNA provided adequate templates for polymerase chain reaction 
      (PCR)-based analyses of several HLA (A, B, C, DRB1, and DQB1) and related loci 
      (HFE, MICA, and 10 microsatellites) in the 6p24.3-6p21.3 region, with PCR 
      amplicons ranging from 92 to 2200 bp. Five different genotyping techniques 
      resolved and confirmed 364 genotypes when both original and Phi29-processed DNA 
      worked in PCRs. General population genetic analyses provided additional evidence 
      that WGA may represent a reliable and simple approach to securing ample genomic 
      DNA for typing HLA, MICA, and related variants.
FAU - Shao, W
AU  - Shao W
AD  - Department of Epidemiology, University of Alabama at Birmingham, Birmingham, AL, 
      USA.
FAU - Tang, J
AU  - Tang J
FAU - Dorak, M T
AU  - Dorak MT
FAU - Song, W
AU  - Song W
FAU - Lobashevsky, E
AU  - Lobashevsky E
FAU - Cobbs, C S
AU  - Cobbs CS
FAU - Wrensch, M R
AU  - Wrensch MR
FAU - Kaslow, R A
AU  - Kaslow RA
LA  - eng
GR  - AI41530/AI/NIAID NIH HHS/United States
GR  - AI41951/AI/NIAID NIH HHS/United States
GR  - CA097247/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (HLA Antigens)
SB  - IM
MH  - Africa
MH  - Artifacts
MH  - Black People/genetics
MH  - Europe
MH  - *Genome, Human
MH  - Genomics/*methods/standards
MH  - HLA Antigens/*genetics
MH  - Humans
MH  - *Polymorphism, Genetic
MH  - Reproducibility of Results
MH  - White People/genetics
EDAT- 2004/08/12 05:00
MHDA- 2005/03/08 09:00
CRDT- 2004/08/12 05:00
PHST- 2004/08/12 05:00 [pubmed]
PHST- 2005/03/08 09:00 [medline]
PHST- 2004/08/12 05:00 [entrez]
AID - TAN295 [pii]
AID - 10.1111/j.0001-2815.2004.00295.x [doi]
PST - ppublish
SO  - Tissue Antigens. 2004 Sep;64(3):286-92. doi: 10.1111/j.0001-2815.2004.00295.x.