PMID- 1530581
OWN - NLM
STAT- MEDLINE
DCOM- 19921019
LR  - 20190501
IS  - 0264-6021 (Print)
IS  - 1470-8728 (Electronic)
IS  - 0264-6021 (Linking)
VI  - 286 ( Pt 2)
IP  - Pt 2
DP  - 1992 Sep 1
TI  - 5,6-Dihydroxyindole-2-carboxylic acid is incorporated in mammalian melanin.
PG  - 491-5
AB  - The role of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) in the biosynthesis of 
      melanins has been studied by using the incorporation of specifically 
      radiolabelled melanogenic precursors into melanins formed by melanocytes growing 
      in vitro and in vivo. Extracts of mouse melanocytes and intact viable melanocytes 
      were found to incorporate into melanin from 25% to more than 60% of 
      [1-14C]tyrosine. Melanins from melanoma tumours grown in mice were radiolabelled 
      with 3,4-dihydroxy[1-14C]phenylalanine, purified and chemoselectively 
      decarboxylated. Determination of the 14CO2 evolved showed that at least 20% of 
      the precursor incorporated in vivo retains the label in the form of 
      non-aminoacidic aromatic-type carboxyl groups. These results provide the first 
      unambiguous demonstration that DHICA is incorporated in physiologically relevant 
      amounts in mammalian melanins.
FAU - Tsukamoto, K
AU  - Tsukamoto K
AD  - Laboratory of Cell Biology, National Cancer Institute, National Institutes of 
      Health, Bethesda, MD 20892.
FAU - Palumbo, A
AU  - Palumbo A
FAU - D'Ischia, M
AU  - D'Ischia M
FAU - Hearing, V J
AU  - Hearing VJ
FAU - Prota, G
AU  - Prota G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (Indoles)
RN  - 0 (Melanins)
RN  - 42HK56048U (Tyrosine)
RN  - 4790-08-3 (5,6-dihydroxy-2-indolylcarboxylic acid)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Indoles/*metabolism
MH  - Melanins/*metabolism
MH  - Melanocytes/metabolism
MH  - Melanoma
MH  - Mice
MH  - Tumor Cells, Cultured
MH  - Tyrosine/metabolism
PMC - PMC1132924
EDAT- 1992/09/01 00:00
MHDA- 1992/09/01 00:01
PMCR- 1992/09/01
CRDT- 1992/09/01 00:00
PHST- 1992/09/01 00:00 [pubmed]
PHST- 1992/09/01 00:01 [medline]
PHST- 1992/09/01 00:00 [entrez]
PHST- 1992/09/01 00:00 [pmc-release]
AID - 10.1042/bj2860491 [doi]
PST - ppublish
SO  - Biochem J. 1992 Sep 1;286 ( Pt 2)(Pt 2):491-5. doi: 10.1042/bj2860491.