PMID- 15306244
OWN - NLM
STAT- MEDLINE
DCOM- 20041104
LR  - 20061115
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 1019
IP  - 1-2
DP  - 2004 Sep 3
TI  - Effects of lipopolysaccharide on the radiation-induced changes in the blood-brain 
      barrier and the astrocytes.
PG  - 105-12
AB  - The use of radiation to improve the efficacy of chemotherapy on malignant brain 
      tumors is also known to cause side effects on vascular endothelial cells and 
      astrocytes in normal parts of the brain. We investigated the effects of 
      lipopolysaccharide (LPS) on the functional and structural properties of 
      blood-brain barrier (BBB) and the activity of astrocytes during whole-brain 
      irradiation in rats. The permeability of the BBB to Evans blue (EB) dye 
      significantly increased in the cerebral cortex, diencephalon and cerebellum 
      regions of rats exposed to irradiation (P<0.01). In contrast, the BBB 
      permeability in irradiated rats was significantly reduced by LPS (P<0.05). Tumor 
      necrosis factor-alpha (TNF-alpha) levels were increased following LPS, 
      irradiation and irradiation plus LPS (P<0.05, P<0.01). Irradiated brain vessels 
      showed a considerable loss of staining intensity of tight junction proteins 
      Zonula occludens-1 (ZO-1) and occludin. Staining for Zonula occludens-1 and 
      occludin was intensive in animals treated with LPS and irradiation plus LPS. 
      Glial fibrillary acidic protein (GFAP) immunoreactivity was seen in very few 
      astrocytes of irradiated brains. However, this staining showed an increased 
      positive intensity in the brain sections of LPS-treated as well as of irradiation 
      plus LPS-treated animals. These results indicate that LPS reduces the passage of 
      exogenous vascular tracer EB-binding albumin into the brain, at least partly, by 
      increasing the expression of tight junction proteins and GFAP, following the 
      irradiation. We suggest that irradiation may affect paracellular permeability 
      through disruption of tight junction proteins, Zonula occludens-1 and occludin, 
      and LPS could provide beneficial effects on the BBB integrity and the astrocytes 
      against irradiation damage.
FAU - Kaya, Mehmet
AU  - Kaya M
AD  - Department of Physiology (Fizyoloji), Istanbul Faculty of Medicine, Istanbul 
      University, Capa 34 390 Istanbul, Turkey. mehkaya@istambul.edu.tr
FAU - Palanduz, Ayse
AU  - Palanduz A
FAU - Kalayci, Rivaze
AU  - Kalayci R
FAU - Kemikler, Gonul
AU  - Kemikler G
FAU - Simsek, Gonul
AU  - Simsek G
FAU - Bilgic, Bilge
AU  - Bilgic B
FAU - Ahishali, Bulent
AU  - Ahishali B
FAU - Arican, Nadir
AU  - Arican N
FAU - Kocyildiz, Zuhal Celebi
AU  - Kocyildiz ZC
FAU - Elmas, Imdat
AU  - Elmas I
FAU - Kucuk, Mutlu
AU  - Kucuk M
FAU - Karadeniz, Ahmet
AU  - Karadeniz A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Membrane Proteins)
SB  - IM
MH  - Animals
MH  - Astrocytes/*drug effects/metabolism/*radiation effects
MH  - Blood-Brain Barrier/*drug effects/metabolism/*radiation effects
MH  - Lipopolysaccharides/*pharmacology
MH  - Male
MH  - Membrane Proteins/metabolism
MH  - Rats
MH  - Rats, Wistar
EDAT- 2004/08/13 05:00
MHDA- 2004/11/05 09:00
CRDT- 2004/08/13 05:00
PHST- 2004/05/25 00:00 [accepted]
PHST- 2004/08/13 05:00 [pubmed]
PHST- 2004/11/05 09:00 [medline]
PHST- 2004/08/13 05:00 [entrez]
AID - S0006899304008972 [pii]
AID - 10.1016/j.brainres.2004.05.102 [doi]
PST - ppublish
SO  - Brain Res. 2004 Sep 3;1019(1-2):105-12. doi: 10.1016/j.brainres.2004.05.102.