PMID- 15307452
OWN - NLM
STAT- MEDLINE
DCOM- 20041026
LR  - 20190507
IS  - 0256-4947 (Print)
IS  - 0975-4466 (Electronic)
IS  - 0256-4947 (Linking)
VI  - 24
IP  - 3
DP  - 2004 May-Jun
TI  - Tissue microarrays for high-throughput molecular pathology.
PG  - 169-74
AB  - Modern research technologies, including DNA, protein, and antibody microarrays 
      identify a steadily growing number of clues that are useful in molecular disease 
      classification, drug development, and the prediction of response to treatment. 
      Subsequent validation of the clinical importance of such candidate genes or 
      proteins requires large-scale analysis of human tissues. To date, this analysis 
      constitutes an important bottleneck in the process of discovery because tissue 
      analysis by the conventional slide-by-slide strategy is slow and expensive. To 
      overcome these limitations, tissue microarray (TMA) technology has been 
      developed. TMA allows for the simultaneous analysis of up to 1,000 tissue samples 
      in a single experiment, using all types of in-situ analyses including 
      immunohistochemistry (IHC), fluorescence in situ hybridization (FISH), and RNA in 
      situ hybridization (RNA-ISH). TMA technology has the potential to greatly 
      facilitate the translation of basic research into clinical practice. Potential 
      applications include the establishment of associations between molecular changes 
      and clinical endpoints, testing of potential therapeutic targets using tissue 
      samples from specific cancer patients, standardization of molecular detection of 
      targets, and rapid translation of results from cell lines and animal models to 
      human cancer. Because of its beneficial economic aspects and ability to 
      differentiate ethnic differences in tumor biology, TMA applications may become 
      particularly important in developing countries.
FAU - Al Kuraya, Khawla
AU  - Al Kuraya K
AD  - King Faisal Hospital and Research Center, Riyadh, Saudi Arabia.
FAU - Simon, Ronald
AU  - Simon R
FAU - Sauter, Guido
AU  - Sauter G
LA  - eng
PT  - Journal Article
PT  - Review
PL  - Saudi Arabia
TA  - Ann Saudi Med
JT  - Annals of Saudi medicine
JID - 8507355
SB  - IM
MH  - *Developing Countries
MH  - Forecasting
MH  - Humans
MH  - Immunohistochemistry/*methods/statistics & numerical data/trends
MH  - Oligonucleotide Array Sequence Analysis/*methods/statistics & numerical 
      data/trends
MH  - Protein Array Analysis/*methods/statistics & numerical data/trends
MH  - Selection Bias
MH  - Specimen Handling
PMC - PMC6147943
EDAT- 2004/08/17 10:00
MHDA- 2004/10/27 09:00
PMCR- 2004/05/01
CRDT- 2004/08/17 10:00
PHST- 2004/08/17 10:00 [pubmed]
PHST- 2004/10/27 09:00 [medline]
PHST- 2004/08/17 10:00 [entrez]
PHST- 2004/05/01 00:00 [pmc-release]
AID - asm-3-169 [pii]
AID - 10.5144/0256-4947.2004.169 [doi]
PST - ppublish
SO  - Ann Saudi Med. 2004 May-Jun;24(3):169-74. doi: 10.5144/0256-4947.2004.169.