PMID- 15308013
OWN - NLM
STAT- MEDLINE
DCOM- 20090507
LR  - 20040813
IS  - 0253-2727 (Print)
IS  - 0253-2727 (Linking)
VI  - 25
IP  - 6
DP  - 2004 Jun
TI  - [The application of fluorescence in situ hybridization to diagnosis of acute 
      promyelocytic leukemia].
PG  - 346-50
AB  - OBJECTIVES: To explore whether PML/RAR alpha fusion gene presented in patients 
      with typical clinical characteristics of acute promyelocytic leukemia (APL) but 
      normal karyotype or atypical translocation of chromosomes 15 and 17 by 
      conventional cytogenetic analysis (CCA), and to assess the application of 
      fluorescence in situ hybridization (FISH) to diagnosis of APL. METHODS: 193 newly 
      diagnosed APL patients received CCA in our hospital, 32 cases of whom were 
      carried out FISH analysis, and some of the patients received reverse 
      transcriptase polymerase chain reaction (RT-PCR) detection. RESULTS: 132 of 193 
      (68.4%) cases were identified to have t(15;17) (q22;q12) by CCA. The selected 32 
      patients were divided into three groups according to CAA results: group 1 
      included 14 cases with typical t(15;17), group 2 included 13 cases without 
      t(15;17), and group 3 included five cases with complex karyotype involving 
      chromosomes 15 and 17. As expected, all cases in group 1 were detected PML/RAR 
      alpha fusion by FISH. In group 2, all patients presented the same molecular 
      abnormality by FISH in spite of absence of t(15;17), and in group 3, FISH not 
      only detected PML/RAR alpha fusion but also identified the fusion signals located 
      on chromosomes, other than chromosome 15q. CONCLUSION: All the APL with typical 
      clinical characteristics can be detected PML/RAR alpha fusion by FISH or RT-PCR 
      regardless of classical t(15;17). FISH is more sensitive for molecularly 
      diagnosis of APL, and can identify the precise location of the fusion signals in 
      complex karyotype. It is necessary in clinically APL patients with no or atypical 
      chromosomal abnormalities to perform FISH analysis.
FAU - Li, Cheng-Wen
AU  - Li CW
AD  - Institute of Hematology and Blood Disease Hospital, CAMS & PUMC, Tianjin 300020, 
      China.
FAU - Liu, Shi-He
AU  - Liu SH
FAU - Bo, Li-Jin
AU  - Bo LJ
FAU - Qin, Shuang
AU  - Qin S
FAU - Liu, Xu-Ping
AU  - Liu XP
FAU - Dai, Yun
AU  - Dai Y
FAU - Wang, Jian-Xiang
AU  - Wang JX
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Xue Ye Xue Za Zhi
JT  - Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi
JID - 8212398
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Child
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Leukemia, Promyelocytic, Acute/*diagnosis
MH  - Male
MH  - Middle Aged
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2004/08/17 10:00
MHDA- 2009/05/08 09:00
CRDT- 2004/08/17 10:00
PHST- 2004/08/17 10:00 [pubmed]
PHST- 2009/05/08 09:00 [medline]
PHST- 2004/08/17 10:00 [entrez]
PST - ppublish
SO  - Zhonghua Xue Ye Xue Za Zhi. 2004 Jun;25(6):346-50.