PMID- 15310064
OWN - NLM
STAT- MEDLINE
DCOM- 20041104
LR  - 20200930
IS  - 0176-1617 (Print)
IS  - 0176-1617 (Linking)
VI  - 161
IP  - 7
DP  - 2004 Jul
TI  - Expression and epitope analysis of the major allergenic protein Fag e 1 from 
      buckwheat.
PG  - 761-7
AB  - Seeds of common buckwheat (Fagopyrum esculentum) contain valuable nutritive 
      substances but also allergenic proteins that cause hypersensitive reactions. 
      Thus, the development of hypoallergenic buckwheat would make this important 
      pseudo-cereal available to allergic people. A major allergenic protein of 
      buckwheat is Fag e 1. We isolated the respective cDNA, coding for a 22 kDa 
      protein, from a recently developed autogamous strain of common buckwheat and 
      confirmed its immunoglobulin E (IgE)-binding activity using recombinant Fag e 1 
      and sera of allergic patients. The derived amino acid sequence from Fag e 1 cDNA 
      was used to synthesize an overlapping peptide library on nitrocellulose membranes 
      for the determination of the Fag e 1 epitopes. We identified eight epitopes and 
      the critical amino acids for IgE-binding within the epitopes. This epitope 
      analysis of a major allergenic protein of buckwheat should help therapeutic 
      efforts and aid in the development of hypoallergenic buckwheat.
FAU - Yoshioka, Hiroyuki
AU  - Yoshioka H
AD  - Graduate School of Agricultural and Biological Sciences, Osaka Prefecture 
      University, Gakuen-cho, Sakai, Osaka, Japan.
FAU - Ohmoto, Tsuyoshi
AU  - Ohmoto T
FAU - Urisu, Atsuo
AU  - Urisu A
FAU - Mine, Yoshinori
AU  - Mine Y
FAU - Adachi, Taiji
AU  - Adachi T
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - J Plant Physiol
JT  - Journal of plant physiology
JID - 9882059
RN  - 0 (Allergens)
RN  - 0 (Antigens, Plant)
RN  - 0 (DNA, Complementary)
RN  - 0 (Epitopes)
RN  - 0 (Fag e 1 allergen, Fagopyrum esculentum)
RN  - 0 (Lymphokines)
RN  - 0 (Plant Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (immunoglobulin-binding factors)
SB  - IM
MH  - Allergens/*genetics/metabolism
MH  - Amino Acid Sequence
MH  - Antigens, Plant
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA, Complementary/chemistry/genetics
MH  - Epitopes/*genetics/metabolism
MH  - Fagopyrum/*genetics/metabolism
MH  - Gene Expression Regulation, Plant/genetics/immunology
MH  - Lymphokines/genetics/metabolism
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Plant Proteins/genetics/metabolism
MH  - Recombinant Proteins/genetics/metabolism
MH  - Sequence Analysis, DNA
EDAT- 2004/08/18 05:00
MHDA- 2004/11/05 09:00
CRDT- 2004/08/18 05:00
PHST- 2004/08/18 05:00 [pubmed]
PHST- 2004/11/05 09:00 [medline]
PHST- 2004/08/18 05:00 [entrez]
AID - S0176-1617(04)00033-1 [pii]
AID - 10.1016/j.jplph.2004.01.010 [doi]
PST - ppublish
SO  - J Plant Physiol. 2004 Jul;161(7):761-7. doi: 10.1016/j.jplph.2004.01.010.