PMID- 15310931
OWN - NLM
STAT- MEDLINE
DCOM- 20041208
LR  - 20190901
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 286
DP  - 2005
TI  - Fluorescence in situ hybridization to localize transgenes in plant chromosomes.
PG  - 327-40
AB  - Production of transgenic plants is now routine for many of our crop species. 
      Methods for the detailed molecular analysis of transgenic plants are available, 
      but often the exact location of the transgene within the crop genome is poorly 
      understood. As a starting point to understanding more about the site of transgene 
      insertion, transgenes can be physically located using fluorescence in situ 
      hybridization (FISH). This technique allows transgenes to be located to specific 
      chromosome regions following the hybridization of a fluorescent labelled probe to 
      a chromosome spread. The technique is sensitive enough to detect single transgene 
      copies and can reveal information about the complexity of a transgene insertion 
      site as well as identifying plants homozygous for the transgene. A FISH method is 
      described that has been used successfully to detect single-transgene copies in 
      mitotic metaphase chromosome preparations of wheat and barley.
FAU - Harwood, Wendy A
AU  - Harwood WA
AD  - Departent of Crop Genetics, John Innes Centre, Norwich, UK.
FAU - Bilham, Lorelei J
AU  - Bilham LJ
FAU - Travella, Silvia
AU  - Travella S
FAU - Salvo-Garrido, Haroldo
AU  - Salvo-Garrido H
FAU - Snape, John W
AU  - Snape JW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Chromosome Mapping/methods
MH  - Chromosomes, Plant/*genetics
MH  - Hordeum/*genetics
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Mitosis
MH  - Plants, Genetically Modified/cytology/*genetics
MH  - Plasmids/genetics
MH  - Triticum/*genetics
EDAT- 2004/08/18 05:00
MHDA- 2004/12/16 09:00
CRDT- 2004/08/18 05:00
PHST- 2004/08/18 05:00 [pubmed]
PHST- 2004/12/16 09:00 [medline]
PHST- 2004/08/18 05:00 [entrez]
AID - 1-59259-827-7:327 [pii]
AID - 10.1385/1-59259-827-7:327 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2005;286:327-40. doi: 10.1385/1-59259-827-7:327.