PMID- 1531292
OWN - NLM
STAT- MEDLINE
DCOM- 19920311
LR  - 20210514
IS  - 0264-6021 (Print)
IS  - 1470-8728 (Electronic)
IS  - 0264-6021 (Linking)
VI  - 281 ( Pt 2)
IP  - Pt 2
DP  - 1992 Jan 15
TI  - Effect of interferon-gamma on complement gene expression in different cell types.
PG  - 437-42
AB  - We have studied the expression of the complement components C2, C3, factor B, C1 
      inhibitor (C1-inh), C4-binding protein (C4-bp) and factor H in human peripheral 
      blood monocytes, skin fibroblasts, umbilical vein endothelial cells (HUVEC) and 
      the human hepatoma cell line G2 (Hep G2) in the absence and the presence of 
      interferon-gamma (IFN-gamma). E.l.i.s.a. performed on culture fluids, run-on 
      transcription assays, Northern blot and double-dilution dot-blot techniques 
      confirmed that monocytes expressed all six components, whereas fibroblasts, HUVEC 
      and HepG2 each expressed five of the six components. Fibroblasts and HUVEC did 
      not synthesize C4-bp, and Hep G2 did not produce factor H. In addition to these 
      differences, the synthesis rates of C3, C1-inh and factor H were not the same in 
      all cell types. However, the synthesis rates of C2 and factor B were similar in 
      all four cell types. The half-lives of the mRNAs were shorter in monocytes than 
      in other cell types. Monocyte factor H mRNA had a half-life of 12 min in 
      monocytes, compared with over 3 h in fibroblasts and HUVEC. The instability of 
      factor H mRNA in monocytes may contribute to their low factor H secretion rate. 
      IFN-gamma produced dose-dependent stimulation of C2, factor B, C1-inh, C4-bp and 
      factor H synthesis by all cell types expressing these proteins, but decreased C3 
      synthesis in all four cell types. Cell-specific differences in the response to 
      IFN-gamma were observed. The increased rates of transcription of the C1-inh and 
      factor H genes in HUVEC were greater than in other cell types, while the 
      increased rate of transcription of the C2, factor B and C1-inh genes in Hep G2 
      cells was less than in other cell types. IFN-gamma did not affect the stability 
      of C3, factor H or C4 bp mRNAs, but increased the stability of factor B and 
      C1-inh mRNAs and decreased the stability of C2 mRNA. Although these changes 
      occurred in all four cell types studied, the half-life of C1-inh mRNA in 
      monocytes was increased almost 4-fold, whereas the increases in the other cell 
      types were less than 30%. These data show that the constitutive synthesis rates 
      of complement components may vary in the different cell types. They also show 
      that the degree of change in synthesis rates in response to IFN-gamma in each of 
      the cell types often varies due to differences in transcriptional response, 
      sometimes in association with changes in mRNA stability.
FAU - Lappin, D F
AU  - Lappin DF
AD  - Department of Pathology, Western Infirmary, Glasgow, U.K.
FAU - Guc, D
AU  - Guc D
FAU - Hill, A
AU  - Hill A
FAU - McShane, T
AU  - McShane T
FAU - Whaley, K
AU  - Whaley K
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (CFH protein, human)
RN  - 0 (Carrier Proteins)
RN  - 0 (Complement C1 Inactivator Proteins)
RN  - 0 (Complement C2)
RN  - 0 (Complement C3)
RN  - 0 (Complement C3b Inactivator Proteins)
RN  - 0 (Complement C4)
RN  - 0 (Integrin alphaXbeta2)
RN  - 0 (RNA, Messenger)
RN  - 80295-43-8 (Complement C3b)
RN  - 80295-65-4 (Complement Factor H)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 9007-36-7 (Complement System Proteins)
SB  - IM
MH  - Carrier Proteins/genetics/metabolism
MH  - Cells, Cultured
MH  - Complement C1 Inactivator Proteins/genetics/metabolism
MH  - Complement C2/genetics/metabolism
MH  - Complement C3/genetics/metabolism
MH  - Complement C3b/genetics/metabolism
MH  - Complement C3b Inactivator Proteins/genetics/metabolism
MH  - Complement C4/metabolism
MH  - Complement Factor H
MH  - Complement System Proteins/*genetics/metabolism
MH  - *Gene Expression Regulation
MH  - Humans
MH  - Integrin alphaXbeta2
MH  - Interferon-gamma/*physiology
MH  - Kinetics
MH  - Organ Specificity/genetics
MH  - RNA, Messenger/metabolism
MH  - Transcription, Genetic
PMC - PMC1130704
EDAT- 1992/01/15 00:00
MHDA- 1992/01/15 00:01
PMCR- 1992/01/15
CRDT- 1992/01/15 00:00
PHST- 1992/01/15 00:00 [pubmed]
PHST- 1992/01/15 00:01 [medline]
PHST- 1992/01/15 00:00 [entrez]
PHST- 1992/01/15 00:00 [pmc-release]
AID - 10.1042/bj2810437 [doi]
PST - ppublish
SO  - Biochem J. 1992 Jan 15;281 ( Pt 2)(Pt 2):437-42. doi: 10.1042/bj2810437.