PMID- 15312963
OWN - NLM
STAT- MEDLINE
DCOM- 20041221
LR  - 20101118
IS  - 0197-4580 (Print)
IS  - 0197-4580 (Linking)
VI  - 25
IP  - 9
DP  - 2004 Oct
TI  - Novel aspects of accumulation dynamics and A beta composition in transgenic 
      models of AD.
PG  - 1175-85
AB  - A homogeneous time-resolved fluorescence immunoassay for detection of 
      beta-amyloid (A beta) peptides has been adapted for quantification of A beta(40) 
      and A beta(42) accumulation in brains of APP695SWE transgenic mice. These 
      over-express human beta APP(swe), beta-amyloid precursor protein (beta-APP) 
      containing the K670N/M671L 'Swedish' familial Alzheimer's disease (FAD) mutation. 
      Both peptides start to accumulate in this line from about 260 to 280 days of age. 
      Co-expression of a human presenilin-1 (PS1) transgene containing the A246E FAD 
      mutation accelerates deposition and also favors-at least initially-accumulation 
      of A beta(42) so that the A beta(2):A beta(40) ratio of peptides from 7- to 
      12-month-old APP695SWE x PS1A246E animals is significantly elevated above that 
      observed throughout the lifetime of APP695SWE mice. These findings, supported by 
      parallel immunohistochemical staining and surface-enhanced laser 
      desorption/ionization time-of-flight (SELDI-TOF) mass spectrometry data, offer 
      important longitudinal characterization of two mouse models of cerebral 
      amyloidosis. Application of the same extraction and quantitation procedures to 
      samples of temporal cortex from AD sufferers indicates however that A beta(40) is 
      only a minor component of beta-amyloid in humans.
FAU - Lewis, Huw D
AU  - Lewis HD
AD  - Department of Molecular and Cellular Neuroscience, Merck Sharp & Dohme Research 
      Laboratories, Neuroscience Research Centre, Terlings Park, Harlow, Essex CM20 
      2QR, UK.
FAU - Beher, Dirk
AU  - Beher D
FAU - Smith, David
AU  - Smith D
FAU - Hewson, Louise
AU  - Hewson L
FAU - Cookson, Natalie
AU  - Cookson N
FAU - Reynolds, David S
AU  - Reynolds DS
FAU - Dawson, Gerard R
AU  - Dawson GR
FAU - Jiang, Michael
AU  - Jiang M
FAU - Van der Ploeg, Lex H T
AU  - Van der Ploeg LH
FAU - Qian, Su
AU  - Qian S
FAU - Rosahl, Thomas W
AU  - Rosahl TW
FAU - Kalaria, Raj N
AU  - Kalaria RN
FAU - Shearman, Mark S
AU  - Shearman MS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Neurobiol Aging
JT  - Neurobiology of aging
JID - 8100437
RN  - 0 (Amyloid beta-Peptides)
RN  - 0 (Amyloid beta-Protein Precursor)
RN  - 0 (Membrane Proteins)
RN  - 0 (PSEN1 protein, human)
RN  - 0 (Peptide Fragments)
RN  - 0 (Presenilin-1)
RN  - 0 (amyloid beta-protein (1-40))
RN  - 0 (amyloid beta-protein (1-42))
SB  - IM
MH  - Aging/genetics/metabolism/pathology
MH  - Alzheimer Disease/genetics/*metabolism/physiopathology
MH  - Amyloid beta-Peptides/*analysis/genetics/metabolism
MH  - Amyloid beta-Protein Precursor/metabolism
MH  - Animals
MH  - Brain/*metabolism/pathology/physiopathology
MH  - Disease Models, Animal
MH  - Disease Progression
MH  - Fluorescence Polarization Immunoassay/methods
MH  - Gliosis/genetics/metabolism/physiopathology
MH  - Humans
MH  - Membrane Proteins/genetics/metabolism
MH  - Mice
MH  - Mice, Transgenic
MH  - Mutation/genetics
MH  - Peptide Fragments/analysis/genetics/metabolism
MH  - Plaque, Amyloid/chemistry/genetics/*metabolism
MH  - Presenilin-1
MH  - Tumor Cells, Cultured
MH  - Up-Regulation/genetics
EDAT- 2004/08/18 05:00
MHDA- 2004/12/22 09:00
CRDT- 2004/08/18 05:00
PHST- 2003/04/16 00:00 [received]
PHST- 2003/10/20 00:00 [revised]
PHST- 2003/12/11 00:00 [accepted]
PHST- 2004/08/18 05:00 [pubmed]
PHST- 2004/12/22 09:00 [medline]
PHST- 2004/08/18 05:00 [entrez]
AID - S0197458004000478 [pii]
AID - 10.1016/j.neurobiolaging.2003.12.009 [doi]
PST - ppublish
SO  - Neurobiol Aging. 2004 Oct;25(9):1175-85. doi: 
      10.1016/j.neurobiolaging.2003.12.009.