PMID- 15313427
OWN - NLM
STAT- MEDLINE
DCOM- 20050223
LR  - 20141120
IS  - 1567-5769 (Print)
IS  - 1567-5769 (Linking)
VI  - 4
IP  - 10-11
DP  - 2004 Oct
TI  - Triacyl-lipopentapeptide adjuvants: TLR2-dependent activation of macrophages and 
      modulation of receptor-mediated cell activation by altering acyl-moieties.
PG  - 1287-300
AB  - Synthetic lipopeptides derived from bacterial lipoprotein are efficient 
      immunoadjuvants. In vitro they activate antigen presenting cells (APCs) to induce 
      the translocation of nuclear factor kappa B (NF-kappaB) and the activation of 
      further transcription factors. This results in the expression of genes encoding 
      cytokines such as IL-1, IL-6, TNF-alpha and in the release of reactive 
      oxygen/nitrogen intermediates. The molecular structure of microbial products 
      determines TLR specificity and thus their activatory potential and 
      immunoadjuvanticity. In the present study, we investigated the 
      lipopeptide-induced activation of leukocytes at different cellular levels by 
      applying derivatives of a synthetic lipopentapeptide-fatty acid library. Our 
      results show that TLR2 plays a key role for the activation of bone marrow-derived 
      macrophages (BMDMs) by lipopentapeptide derivatives and that the fatty acid 
      composition of the lipopeptides determines their activation potential and TLR 
      specificity.
FAU - Muller, Silke D C
AU  - Muller SD
AD  - Institut fur Molekulare Medizin und Zellforschung der Universitat, AK 
      Tumorimmunologie/Vakzine, Stefan-Meier-Str. 8, D-79104 Freiburg, Germany.
FAU - Muller, Markus R
AU  - Muller MR
FAU - Huber, Maria
AU  - Huber M
FAU - Esche Uv, Ulrich v d
AU  - Esche Uv Uv
FAU - Kirschning, Carsten J
AU  - Kirschning CJ
FAU - Wagner, H
AU  - Wagner H
FAU - Bessler, Wolfgang G
AU  - Bessler WG
FAU - Mittenbuhler, Klaus
AU  - Mittenbuhler K
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Int Immunopharmacol
JT  - International immunopharmacology
JID - 100965259
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Bacterial Proteins)
RN  - 0 (Fatty Acids, Unsaturated)
RN  - 0 (Lipoproteins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (NF-kappa B)
RN  - 0 (Peptide Library)
RN  - 0 (Peptides)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (TLR2 protein, human)
RN  - 0 (Tlr2 protein, mouse)
RN  - 0 (Toll-Like Receptor 2)
RN  - 0 (Toll-Like Receptors)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
SB  - IM
MH  - Adjuvants, Immunologic/chemistry/*pharmacology
MH  - Animals
MH  - Bacterial Proteins/chemistry
MH  - Cell Line
MH  - Extracellular Signal-Regulated MAP Kinases/metabolism
MH  - Fatty Acids, Unsaturated/*chemistry
MH  - Humans
MH  - In Vitro Techniques
MH  - Lipoproteins/*chemistry
MH  - Macrophage Activation/genetics/*physiology
MH  - Macrophages/*metabolism
MH  - Membrane Glycoproteins/genetics/physiology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - NF-kappa B/metabolism
MH  - Nitric Oxide/metabolism
MH  - Peptide Library
MH  - Peptides/chemistry/*pharmacology
MH  - Phosphorylation
MH  - Receptors, Cell Surface/genetics/*physiology
MH  - Toll-Like Receptor 2
MH  - Toll-Like Receptors
MH  - Transfection
EDAT- 2004/08/18 05:00
MHDA- 2005/02/24 09:00
CRDT- 2004/08/18 05:00
PHST- 2003/09/11 00:00 [received]
PHST- 2004/02/04 00:00 [revised]
PHST- 2004/04/22 00:00 [accepted]
PHST- 2004/08/18 05:00 [pubmed]
PHST- 2005/02/24 09:00 [medline]
PHST- 2004/08/18 05:00 [entrez]
AID - S1567576904001420 [pii]
AID - 10.1016/j.intimp.2004.04.012 [doi]
PST - ppublish
SO  - Int Immunopharmacol. 2004 Oct;4(10-11):1287-300. doi: 
      10.1016/j.intimp.2004.04.012.