PMID- 15314275
OWN - NLM
STAT- MEDLINE
DCOM- 20050301
LR  - 20131121
IS  - 1389-5729 (Print)
IS  - 1389-5729 (Linking)
VI  - 5
IP  - 4
DP  - 2004
TI  - Effect of estradiol and progesterone treatment on carbohydrate metabolizing 
      enzymes in tissues of aging female rats.
PG  - 249-59
AB  - The aim of this study was to determine the effect of administration of estradiol 
      (E2), progesterone (P4), and combination of estradiol and progesterone (EP) in 
      aging female rats. The changes in the activities of hexokinase (HK), 
      glucose-6-phosphatase (G6P'tase) and glucose-6-phosphate dehydrogenase (G6PDH) 
      enzymes, and in protein levels in tissues of rats namely brain (cerebral 
      hemisphere), heart, liver, kidney and uterus have been measured in different age 
      groups. The random blood sugar level was measured in serum and liver. The 
      different age groups of rats were given 0.1 microg/g body weight estradiol, 2.5 
      microg/g body weight progesterone and a similar concentration of both in a 
      combined treatment for 1 month. This dose was selected after determining estrogen 
      and progesterone levels in 3 month adult female animals so that the aging female 
      animals had circulating hormone levels nearly the same as those of young female 
      animals. The random sugar level was determined in serum and liver cytosolic 
      fractions, and it was increased by combination treatment. The protein content in 
      tissues showed significant changes only with combined hormone administration when 
      compared with age-matched controls. The activity of HK decreased in aged animals 
      and significantly increased by hormone treatments in all the tissues of the aged 
      rats studied. The activity of G6P'tase increased with age up to 1.5 years and 
      decreased in 2 years. Treatment with E2 and EP further decreased the activity 
      significantly in all the tissues. G6PDH showed a similar pattern as was observed 
      in HK in all the age groups. Therefore, the E2 and EP treatments caused an entire 
      series of growth-related responses, including an increased uptake of glucose, 
      increased the protein level in the tissues of aging rats, thereby reducing the 
      risk factors associated with aging by normalizing hormone levels which decreased 
      with aging and resulted in diseases such as Alzheimer's diseases and diabetes.
FAU - Moorthy, K
AU  - Moorthy K
AD  - Hormone and Drug Research Laboratory, School of Life Sciences, Jawaharlal Nehru 
      University, New Delhi 110067, India.
FAU - Yadav, Umesh C S
AU  - Yadav UC
FAU - Siddiqui, M R
AU  - Siddiqui MR
FAU - Sharma, D
AU  - Sharma D
FAU - Basir, S F
AU  - Basir SF
FAU - Baquer, N Z
AU  - Baquer NZ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biogerontology
JT  - Biogerontology
JID - 100930043
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 4TI98Z838E (Estradiol)
RN  - EC 1.1.1.49 (Glucosephosphate Dehydrogenase)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - EC 3.1.3.9 (Glucose-6-Phosphatase)
SB  - IM
MH  - Age Factors
MH  - Aging/drug effects/*physiology
MH  - Animals
MH  - Carbohydrate Metabolism
MH  - Estradiol/administration & dosage/*pharmacokinetics
MH  - Female
MH  - Glucose-6-Phosphatase/drug effects/*metabolism
MH  - Glucosephosphate Dehydrogenase/drug effects/*metabolism
MH  - Hexokinase/drug effects/*metabolism
MH  - Models, Animal
MH  - Progesterone/administration & dosage/*pharmacokinetics
MH  - Rats
MH  - Rats, Wistar
EDAT- 2004/08/18 05:00
MHDA- 2005/03/02 09:00
CRDT- 2004/08/18 05:00
PHST- 2004/08/18 05:00 [pubmed]
PHST- 2005/03/02 09:00 [medline]
PHST- 2004/08/18 05:00 [entrez]
AID - 5276620 [pii]
AID - 10.1023/B:BGEN.0000038026.89337.02 [doi]
PST - ppublish
SO  - Biogerontology. 2004;5(4):249-59. doi: 10.1023/B:BGEN.0000038026.89337.02.