PMID- 15317669
OWN - NLM
STAT- MEDLINE
DCOM- 20050408
LR  - 20220318
IS  - 1073-449X (Print)
IS  - 1073-449X (Linking)
VI  - 170
IP  - 11
DP  - 2004 Dec 1
TI  - Impact of cigarette smoke on clearance and inflammation after Pseudomonas 
      aeruginosa infection.
PG  - 1164-71
AB  - The object of this study was to investigate the impact of cigarette smoke on 
      bacterial clearance and immune inflammatory parameters after infection with 
      Pseudomonas aeruginosa in mice. We observed a delayed rate of bacterial clearance 
      in smoke-exposed compared with sham-exposed mice. This was associated with 
      increased inflammation characterized by greater numbers of neutrophils and 
      mononuclear cells in the bronchoalveolar lavage. After infection, we observed 
      increased levels of proinflammatory cytokines (tumor necrosis factor-alpha, 
      interleukin-1beta, and interleukin-6) and chemokines (monocyte chemoattractant 
      protein-1 [MCP-1] and macrophage inflammatory protein-2 [MIP-2]) as well as 
      myeloperoxidase and proteolytic activity in the lungs of smoke-exposed compared 
      with sham-exposed animals. Delayed clearance was associated with increased 
      morbidity and greater weight loss of smoke-exposed mice. After delivery of 
      inactivated bacteria, we observed a similar inflammatory response, clinical 
      score, and tumor necrosis factor-alpha expression in smoke- and sham-exposed 
      animals, suggesting that increased inflammation and altered clinical presentation 
      are due to the delayed rate of bacterial clearance. Our findings suggest that 
      cigarette smoke affects respiratory immune-inflammatory responses elicited by 
      bacteria. We postulate that altered respiratory host defense may be implicated in 
      smoking-related diseases such as chronic obstructive pulmonary disease.
FAU - Drannik, Anna G
AU  - Drannik AG
AD  - McMaster University, Department of Pathology and Molecular Medicine, Health 
      Sciences Centre, Room 4H21A, 1200 Main Street West, Hamilton, ON, L8N 3Z5 Canada.
FAU - Pouladi, Mahmoud A
AU  - Pouladi MA
FAU - Robbins, Clinton S
AU  - Robbins CS
FAU - Goncharova, Susanna I
AU  - Goncharova SI
FAU - Kianpour, Sussan
AU  - Kianpour S
FAU - Stampfli, Martin R
AU  - Stampfli MR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20040818
PL  - United States
TA  - Am J Respir Crit Care Med
JT  - American journal of respiratory and critical care medicine
JID - 9421642
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
RN  - 0 (Tobacco Smoke Pollution)
RN  - EC 3.4.- (Peptide Hydrolases)
SB  - IM
MH  - Animals
MH  - Bronchoalveolar Lavage Fluid/immunology
MH  - Chemokines/immunology
MH  - Cytokines/immunology
MH  - Female
MH  - Macrophages, Alveolar/immunology
MH  - Mice
MH  - Mucociliary Clearance/*immunology
MH  - Peptide Hydrolases/immunology
MH  - Pneumonia/*immunology/physiopathology
MH  - Pseudomonas Infections/*immunology/physiopathology
MH  - *Pseudomonas aeruginosa
MH  - Time Factors
MH  - Tobacco Smoke Pollution/*adverse effects
EDAT- 2004/08/20 05:00
MHDA- 2005/04/09 09:00
CRDT- 2004/08/20 05:00
PHST- 2004/08/20 05:00 [pubmed]
PHST- 2005/04/09 09:00 [medline]
PHST- 2004/08/20 05:00 [entrez]
AID - 200311-1521OC [pii]
AID - 10.1164/rccm.200311-1521OC [doi]
PST - ppublish
SO  - Am J Respir Crit Care Med. 2004 Dec 1;170(11):1164-71. doi: 
      10.1164/rccm.200311-1521OC. Epub 2004 Aug 18.