PMID- 15321685
OWN - NLM
STAT- MEDLINE
DCOM- 20041119
LR  - 20131121
IS  - 0378-1097 (Print)
IS  - 0378-1097 (Linking)
VI  - 237
IP  - 2
DP  - 2004 Aug 15
TI  - Microdiversity of phenol hydroxylase genes among phenol-degrading isolates of 
      Alcaligenes sp. from an activated sludge system.
PG  - 369-75
AB  - Enterobacterial repetitive intergenic consensus (ERIC)-PCR fingerprinting 
      classified 97 phenol-degrading isolates with identical amplified ribosomal DNA 
      restriction analysis (ARDRA) patterns into six genotypic groups. The 16S rRNA 
      gene of the representative isolate of each group had higher than 99.47% common 
      identity with each other and higher than 98% identity with the type strain of 
      Alcaligenes faecalis. PCR-TGGE (temperature gradient gel electrophoresis) 
      analysis of the genes of the largest subunit of the multi-component phenol 
      hydroxylase (LmPH) in each isolate followed with sequencing showed that isolates 
      within each ERIC-PCR group had identical LmPH gene sequences. Among the six 
      different ERIC-PCR groups, two were found to harbor two different LmPH genes 
      encoding low- and high-Ks (affinity constants) phenol hydroxylases, and the 
      low-Ks type LmPH was identical in sequence with one predominant LmPH of the 
      parental activated sludge. Three ERIC-PCR groups had only the high-Ks type and 
      one had no sequence similar to the known LmPHs. Our work suggests that there is 
      no correlation between the phylogenetic groupings of phenol-degrading bacteria 
      and their LmPH genotypes possibly due to extensive horizontal gene transfer of 
      this functional gene.
FAU - Zhang, Xueli
AU  - Zhang X
AD  - Laboratory of Molecular Microbial Ecology and Ecogenomics, College of Life 
      Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, 
      Shanghai 200240, China.
FAU - Gao, Pingping
AU  - Gao P
FAU - Chao, Qunfang
AU  - Chao Q
FAU - Wang, Linghua
AU  - Wang L
FAU - Senior, Eric
AU  - Senior E
FAU - Zhao, Liping
AU  - Zhao L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEMS Microbiol Lett
JT  - FEMS microbiology letters
JID - 7705721
RN  - 0 (DNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
RN  - 339NCG44TV (Phenol)
RN  - EC 1.- (Mixed Function Oxygenases)
RN  - EC 1.14.13.7 (phenol 2-monooxygenase)
SB  - IM
MH  - Alcaligenes/*enzymology/genetics/isolation & purification
MH  - DNA Fingerprinting
MH  - DNA, Bacterial/analysis
MH  - *Genes, Bacterial
MH  - Genes, rRNA
MH  - Genetic Variation
MH  - Mixed Function Oxygenases/classification/*genetics/metabolism
MH  - Nucleic Acid Hybridization
MH  - Phenol/*metabolism
MH  - Phylogeny
MH  - Polymerase Chain Reaction
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - Sewage/*microbiology
EDAT- 2004/08/24 05:00
MHDA- 2004/12/16 09:00
CRDT- 2004/08/24 05:00
PHST- 2004/04/12 00:00 [received]
PHST- 2004/06/26 00:00 [revised]
PHST- 2004/06/30 00:00 [accepted]
PHST- 2004/08/24 05:00 [pubmed]
PHST- 2004/12/16 09:00 [medline]
PHST- 2004/08/24 05:00 [entrez]
AID - S0378-1097(04)00491-4 [pii]
AID - 10.1016/j.femsle.2004.06.057 [doi]
PST - ppublish
SO  - FEMS Microbiol Lett. 2004 Aug 15;237(2):369-75. doi: 
      10.1016/j.femsle.2004.06.057.