PMID- 15322070
OWN - NLM
STAT- MEDLINE
DCOM- 20050524
LR  - 20220330
IS  - 8750-7587 (Print)
IS  - 0161-7567 (Linking)
VI  - 98
IP  - 2
DP  - 2005 Feb
TI  - Effect of prolonged exercise and carbohydrate ingestion on type 1 and type 2 T 
      lymphocyte distribution and intracellular cytokine production in humans.
PG  - 565-71
AB  - The present study was undertaken to examine the role of the exercise-induced 
      stress hormone response on the regulation of type 1 and type 2 T lymphocyte 
      intracellular cytokine production. Subjects performed 2.5 h of cycling exercise 
      at 65% maximal O2 uptake while ingesting a 6.4% carbohydrate (CHO) solution, 
      12.8% CHO solution, or a placebo. Peripheral whole blood samples were stimulated 
      and stained for T lymphocyte surface antigens (CD4 and CD8). Cells were then 
      permeabilized, stained for intracellular cytokines, and analyzed using flow 
      cytometry. Exercise resulted in a decrease (P < 0.05) in the number and 
      percentage of IFN-gamma positive CD4+ and CD8+ T lymphocytes. These stimulated 
      cells produced less IFN-gamma immediately postexercise (P < 0.05) and 2-h 
      postexercise (P < 0.05) compared with preexercise. However, CHO ingestion, which 
      attenuated the exercise-induced stress hormone response compared with placebo (P 
      < 0.05), prevented both the decrease in the number and percentage of 
      IFN-gamma-positive CD4+ and CD8+ T lymphocytes and the suppression of IFN-gamma 
      production from stimulated CD4+ and CD8+ T lymphocytes. There was no effect of 
      exercise on the number of, or cytokine production from, IL-4-positive CD4+ or 
      CD8+ T lymphocytes. These data provide support for the role of exercise-induced 
      elevations in stress hormones in the regulation of type 1 T lymphocyte cytokine 
      production and distribution.
FAU - Lancaster, G I
AU  - Lancaster GI
AD  - School of Sport and Exercise Sciences, Loughborough Univ., Loughborough LE11 3TU, 
      United Kingdom.
FAU - Khan, Q
AU  - Khan Q
FAU - Drysdale, P T
AU  - Drysdale PT
FAU - Wallace, F
AU  - Wallace F
FAU - Jeukendrup, A E
AU  - Jeukendrup AE
FAU - Drayson, M T
AU  - Drayson MT
FAU - Gleeson, M
AU  - Gleeson M
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
DEP - 20040820
PL  - United States
TA  - J Appl Physiol (1985)
JT  - Journal of applied physiology (Bethesda, Md. : 1985)
JID - 8502536
RN  - 0 (Cytokines)
RN  - 0 (Dietary Carbohydrates)
SB  - IM
MH  - Adaptation, Physiological/physiology
MH  - Adult
MH  - CD4-Positive T-Lymphocytes/*cytology/*metabolism
MH  - CD8-Positive T-Lymphocytes/*cytology/*metabolism
MH  - Cytokines/*blood
MH  - Dietary Carbohydrates/*metabolism
MH  - Exercise Test
MH  - Humans
MH  - Intracellular Space/metabolism
MH  - Male
MH  - Physical Exertion/*physiology
MH  - Tissue Distribution
EDAT- 2004/08/24 05:00
MHDA- 2005/05/25 09:00
CRDT- 2004/08/24 05:00
PHST- 2004/08/24 05:00 [pubmed]
PHST- 2005/05/25 09:00 [medline]
PHST- 2004/08/24 05:00 [entrez]
AID - 00754.2004 [pii]
AID - 10.1152/japplphysiol.00754.2004 [doi]
PST - ppublish
SO  - J Appl Physiol (1985). 2005 Feb;98(2):565-71. doi: 
      10.1152/japplphysiol.00754.2004. Epub 2004 Aug 20.