PMID- 15323147
OWN - NLM
STAT- MEDLINE
DCOM- 20040923
LR  - 20190816
IS  - 0002-9173 (Print)
IS  - 0002-9173 (Linking)
VI  - 122
IP  - 2
DP  - 2004 Aug
TI  - Chromosomal aberration of the 11q23 locus in acute leukemia and frequency of MLL 
      gene translocation: results in 378 adult patients.
PG  - 298-306
AB  - Structural abnormality of the 11q23 band (11q23+) bearing the MLL gene 
      translocation (MLL+) is a recurrent chromosome change observed in 3% to 7% of 
      acute lymphoblastic leukemias and in 3% to 4% of acute myeloblastic leukemias. 
      The resolution of conventional cytogenetics (CC) in detecting 11q23 rearrangement 
      is limited when the translocative partner has a telomeric location; furthermore, 
      CC can barely discriminate between true 11q23+/MLL+ and rearrangements clustering 
      within the 11q22 to approximately 25 region without MLL involvement (MLL-). We 
      characterized a series of 378 consecutive patients with adult acute leukemia by 
      using CC, fluorescence in situ hybridization (FISH), and multiplex karyotyping 
      (M-FISH) analysis. Our aim was to define the frequency of cryptic MLL+ cases and 
      the frequency of MLL+ within 11q22 to approximately 25+ cases. As expected, FISH 
      was more sensitive than CC in detecting MLL+ cases, but rather unexpectedly, 9 
      (45%) of 20 patients with 11q22 to approximately 25+ were MLL-. A better 
      characterization of 11q22 to approximately 25+/MLL- leukemias is relevant for the 
      identification of new, recurrent translocations. Moreover, these cases should be 
      readily distinguishable from 11q23+/MLL+ cases. We recommend that karyotypic 
      analysis always be complemented by molecular or FISH methods to unravel MLL 
      rearrangements.
FAU - Cox, M Christina
AU  - Cox MC
AD  - Department of Hematology, S'Eugenio Hospital, University of Tor Vergata, Rome, 
      Italy.
FAU - Panetta, Paola
AU  - Panetta P
FAU - Lo-Coco, Francesco
AU  - Lo-Coco F
FAU - Del Poeta, Giovanni
AU  - Del Poeta G
FAU - Venditti, Adriano
AU  - Venditti A
FAU - Maurillo, Luca
AU  - Maurillo L
FAU - Del Principe, M Ilaria
AU  - Del Principe MI
FAU - Mauriello, Alessandro
AU  - Mauriello A
FAU - Anemona, Lucia
AU  - Anemona L
FAU - Bruno, Antonio
AU  - Bruno A
FAU - Mazzone, Carla
AU  - Mazzone C
FAU - Palombo, Paolo
AU  - Palombo P
FAU - Amadori, Sergio
AU  - Amadori S
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (KMT2A protein, human)
RN  - 0 (Transcription Factors)
RN  - 149025-06-9 (Myeloid-Lymphoid Leukemia Protein)
RN  - EC 2.1.1.43 (Histone-Lysine N-Methyltransferase)
SB  - IM
MH  - Acute Disease
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Chromosome Aberrations
MH  - Cytodiagnosis/methods
MH  - DNA-Binding Proteins/*genetics
MH  - Female
MH  - Histone-Lysine N-Methyltransferase
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Leukemia/*diagnosis/*genetics
MH  - Male
MH  - Middle Aged
MH  - Myeloid-Lymphoid Leukemia Protein
MH  - *Proto-Oncogenes
MH  - Sensitivity and Specificity
MH  - *Transcription Factors
MH  - *Translocation, Genetic
EDAT- 2004/08/25 05:00
MHDA- 2004/09/24 05:00
CRDT- 2004/08/25 05:00
PHST- 2004/08/25 05:00 [pubmed]
PHST- 2004/09/24 05:00 [medline]
PHST- 2004/08/25 05:00 [entrez]
AID - 10.1309/RX27-R8GJ-QM33-0C22 [doi]
PST - ppublish
SO  - Am J Clin Pathol. 2004 Aug;122(2):298-306. doi: 10.1309/RX27-R8GJ-QM33-0C22.