PMID- 1532337
OWN - NLM
STAT- MEDLINE
DCOM- 19920430
LR  - 20190827
IS  - 0954-7894 (Print)
IS  - 0954-7894 (Linking)
VI  - 22
IP  - 1
DP  - 1992 Jan
TI  - IgE binding inhibits arachidonic acid-induced chemiluminescence of human 
      platelets.
PG  - 91-7
AB  - Arachidonic acid (AA)-induced chemiluminescence (CHL) was studied in vitro by 
      means of a luminometer in platelets from nine healthy volunteers and six allergic 
      patients. The amplitude of the CHL signal increased with AA concentration from 
      250 microM to 7 mM. At a low AA concentration (250 microM), the CHL signal 
      consisted of two peaks. The first one occurred at 6 +/- 3 sec and the second one 
      at 90 +/- 15 sec (n = 9). The mean amplitude of these peaks was 1.95 +/- 0.61 
      mV/sec and 0.82 +/- 0.22 mV/sec for normal subjects, and 2.35 +/- 0.62 mV/sec and 
      0.78 +/- 0.26 mV/sec for allergic patients, respectively. Aspirin (a 
      cyclooxygenase inhibitor) and baicalein (a lipoxygenase inhibitor) reduced in a 
      concentration-dependent manner, the first and second peak, respectively. The 
      binding of immunoglobulin E (IgE) alone to platelets from both normal and 
      allergic subjects inhibited both the first and second peak of AA-induced CHL. 
      This inhibitory effect was specifically due to the action of IgE as it was (i) 
      concentration-dependent and (ii) not observed when immunoglobulin G (IgG) was 
      substituted for IgE. It is concluded that in normal subjects, as well as in 
      allergic patients, the binding of IgE alone to its specific receptor on human 
      platelets could alter arachidonate metabolism that probably involves 
      cyclooxygenase and lipoxygenase pathways.
FAU - Rio, P
AU  - Rio P
AD  - Service des Maladies Respiratoires, Hopital du Haut Leveque, CHR de Bordeaux, 
      Pessac, France.
FAU - Tunon de Lara, J M
AU  - Tunon de Lara JM
FAU - Marthan, R
AU  - Marthan R
FAU - Taytard, A
AU  - Taytard A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Exp Allergy
JT  - Clinical and experimental allergy : journal of the British Society for Allergy 
      and Clinical Immunology
JID - 8906443
RN  - 0 (Antigens, Differentiation, B-Lymphocyte)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Receptors, Fc)
RN  - 0 (Receptors, IgE)
RN  - 27YG812J1I (Arachidonic Acid)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adult
MH  - Antigens, Differentiation, B-Lymphocyte/metabolism
MH  - Arachidonic Acid/pharmacology
MH  - Blood Platelets/drug effects/*immunology
MH  - Female
MH  - Humans
MH  - Hypersensitivity/immunology
MH  - Immunoglobulin E/*blood
MH  - Immunoglobulin G/blood
MH  - In Vitro Techniques
MH  - Luminescent Measurements
MH  - Male
MH  - Receptors, Fc/metabolism
MH  - Receptors, IgE
EDAT- 1992/01/01 00:00
MHDA- 1992/01/01 00:01
CRDT- 1992/01/01 00:00
PHST- 1992/01/01 00:00 [pubmed]
PHST- 1992/01/01 00:01 [medline]
PHST- 1992/01/01 00:00 [entrez]
AID - 10.1111/j.1365-2222.1992.tb00119.x [doi]
PST - ppublish
SO  - Clin Exp Allergy. 1992 Jan;22(1):91-7. doi: 10.1111/j.1365-2222.1992.tb00119.x.