PMID- 1532404
OWN - NLM
STAT- MEDLINE
DCOM- 19920427
LR  - 20170214
IS  - 0022-1554 (Print)
IS  - 0022-1554 (Linking)
VI  - 40
IP  - 4
DP  - 1992 Apr
TI  - Application and comparison of silver intensification methods for the 
      diaminobenzidine and diaminobenzidine-nickel endproduct of the peroxidation 
      reaction in immunohistochemistry and in situ hybridization.
PG  - 495-504
AB  - Silver-intensification methods described in the literature for the 
      diaminobenzidine (DAB) and diaminobenzidine-nickel (DAB/Ni) endproduct of the 
      peroxidase reaction were compared in model systems after immunoperoxidase and in 
      situ hybridization. First, these methods were compared in immunohistochemical 
      model systems, using the demonstration of glial fibrillar acidic protein (GFAP) 
      and prostate-specific antigen (PSA) in paraffin sections of human brain and 
      prostate tissue, respectively. When DAB without Ni was used as substrate, tissue 
      argyrophilia caused considerable background staining. Only when this tissue 
      reactivity was quenched with, e.g., CuSO4 with H2O2 or thioglycolic acid, were 
      the results acceptable. A considerable improvement in the signal-to-noise ratio 
      could be obtained when nickel was included in the substrate mixture. The methods 
      that proved to be best for demonstration of GFAP and PSA made use of acid 
      developer solutions. Subsequently, these methods were compared with other 
      sensitive immunostaining methods for demonstration of the gamma-delta T-cell 
      receptor in frozen lymphoid tissue. In this model a considerable increase in the 
      number of positive cells could be obtained using silver intensification. The 
      different methods using DAB/Ni were also compared for use in DNA in situ 
      hybridization (DISH). In this case two model systems were used: human papilloma 
      virus type 11 (HPV-11) DNA in condyloma tissue (abundant target model) and 
      Epstein-Barr virus (EBV) DNA in a mononucleosis lymph node (low target model). 
      For demonstration of HPV-11, all methods gave more or less satisfactory results, 
      which were best with the acid developer solutions. Moreover, for demonstration of 
      EBV DNA, a signal could be obtained only with these developer solutions. Such a 
      method also proved suitable in double immuno-hybrido stainings for the 
      demonstration of EBV DNA in specific antigen-positive Reed-Sternberg cells in 
      paraffin sections of Hodgkin lymph nodes.
FAU - Mullink, H
AU  - Mullink H
AD  - Institute of Pathology, Free University Hospital, Amsterdam, The Netherlands.
FAU - Vos, W
AU  - Vos W
FAU - Jiwa, M
AU  - Jiwa M
FAU - Horstman, A
AU  - Horstman A
FAU - van der Valk, P
AU  - van der Valk P
FAU - Walboomers, J M
AU  - Walboomers JM
FAU - Meijer, C J
AU  - Meijer CJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Histochem Cytochem
JT  - The journal of histochemistry and cytochemistry : official journal of the 
      Histochemistry Society
JID - 9815334
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (DNA, Viral)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Receptors, Antigen, T-Cell, gamma-delta)
RN  - 2RV4T6KHQI (3,3'-Diaminobenzidine)
RN  - 7OV03QG267 (Nickel)
RN  - EC 1.11.1.- (Horseradish Peroxidase)
SB  - IM
MH  - *3,3'-Diaminobenzidine
MH  - Biomarkers, Tumor/analysis
MH  - DNA, Viral/analysis
MH  - Glial Fibrillary Acidic Protein/analysis
MH  - Horseradish Peroxidase
MH  - Humans
MH  - *Immunoenzyme Techniques
MH  - Lymph Nodes/pathology
MH  - Male
MH  - *Nickel
MH  - *Nucleic Acid Hybridization
MH  - Receptors, Antigen, T-Cell, gamma-delta/analysis
MH  - *Silver Staining
EDAT- 1992/04/01 00:00
MHDA- 1992/04/01 00:01
CRDT- 1992/04/01 00:00
PHST- 1992/04/01 00:00 [pubmed]
PHST- 1992/04/01 00:01 [medline]
PHST- 1992/04/01 00:00 [entrez]
AID - 10.1177/40.4.1532404 [doi]
PST - ppublish
SO  - J Histochem Cytochem. 1992 Apr;40(4):495-504. doi: 10.1177/40.4.1532404.