PMID- 15325083
OWN - NLM
STAT- MEDLINE
DCOM- 20050310
LR  - 20061115
IS  - 0168-1702 (Print)
IS  - 0168-1702 (Linking)
VI  - 105
IP  - 1
DP  - 2004 Sep 15
TI  - Characterization of heteroclite subgenomic RNAs associated with PRRSV infection.
PG  - 75-87
AB  - In this study, porcine reproductive and respiratory syndrome virus (PRRSV) 
      heteroclite (uncommon forms) RNAs were characterized. Nucleotide sequencing of 11 
      additional defective RNA species verified that heteroclites are formed between 
      the 5' and 3' termini of PRRSV at short stretches of identity, with variability 
      seen between the junction sites utilized. Northern blot and RT-PCR analyses 
      indicated that heteroclite RNA species were likely to be packaged into purified 
      virions. To study whether heteroclite RNAs and viral genomic RNAs could be 
      packaged into the same virions, PRRSV strain VR-2332 was purified by sucrose 
      density gradient centrifugation. RT-PCR amplification of the viral RNAs isolated 
      from three distinct gradient bands, using genomic- and heteroclite-specific 
      primer pairs, demonstrated that heteroclite RNAs could not be readily dissociated 
      from genomic RNA. Partial segregation of full-length and larger heteroclite 
      genomes to the upper two gradient bands was seen, but smaller species could be 
      found in all three fractions. These results strongly suggest that heteroclite 
      RNAs retain the PRRSV RNA packaging signal. In vitro transcription and 
      translation of one heteroclite cDNA clone verified that the RNA could express a 
      predicted 32.6 kDa protein, indicating that these RNA species have the potential 
      to produce abnormal proteins in infected cells.
FAU - Yuan, Shishan
AU  - Yuan S
AD  - Department of Veterinary PathoBiology, University of Minnesota, 205 Veterinary 
      Science Building, 1971 Commonwealth Avenue, St. Paul, MN 55108, USA.
FAU - Murtaugh, Michael P
AU  - Murtaugh MP
FAU - Schumann, Faith A
AU  - Schumann FA
FAU - Mickelson, Dan
AU  - Mickelson D
FAU - Faaberg, Kay S
AU  - Faaberg KS
LA  - eng
SI  - GENBANK/AY256685
SI  - GENBANK/AY256686
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Cell Line
MH  - Centrifugation, Density Gradient
MH  - DNA, Complementary/chemistry
MH  - Haplorhini
MH  - Molecular Sequence Data
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*isolation & 
      purification/*physiology
MH  - RNA, Viral/*genetics/*isolation & purification/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Analysis, DNA
MH  - Terminal Repeat Sequences
MH  - Transcription, Genetic
MH  - Virion/*chemistry
MH  - Virus Assembly
EDAT- 2004/08/25 05:00
MHDA- 2005/03/11 09:00
CRDT- 2004/08/25 05:00
PHST- 2003/12/16 00:00 [received]
PHST- 2004/04/21 00:00 [revised]
PHST- 2004/04/21 00:00 [accepted]
PHST- 2004/08/25 05:00 [pubmed]
PHST- 2005/03/11 09:00 [medline]
PHST- 2004/08/25 05:00 [entrez]
AID - S0168170204002229 [pii]
AID - 10.1016/j.virusres.2004.04.015 [doi]
PST - ppublish
SO  - Virus Res. 2004 Sep 15;105(1):75-87. doi: 10.1016/j.virusres.2004.04.015.