PMID- 15325094
OWN - NLM
STAT- MEDLINE
DCOM- 20040930
LR  - 20071115
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 153
IP  - 1
DP  - 2004 Aug
TI  - Determination of secondary chromosomal aberrations of chronic myelocytic 
      leukemia.
PG  - 53-6
AB  - Chronic myeloctyic leukemia (CML) is a stem cell disorder characterized by the 
      cytogenetic abnormality of t(9;22)(q34;q11.2), which progresses from a chronic 
      phase to an accelerated phase (AP), and/or a blast phase (BP) of myelocytic or 
      lymphoid phenotype. This progression is frequently preceded or accompanied by 
      recurring secondary chromosomal abnormalities (SCA) that are believed to play a 
      role in the transformation and may also serve as valuable prognostic indicators. 
      Failure to note such abnormalities may lead to an inappropriate clinical 
      evaluation. We observed CML patients with AP or BP who did not show SCA by 
      routine cytogenetic analysis. To determine the presence or absence of specific 
      SCA in those cases, we applied fluorescence in situ hybridization (FISH) to four 
      CML cases with pseudodiploid cytogenetics [t(9;22)(q34;11.2) as the sole 
      abnormality] by conventional karyotyping. Bone marrow biopsies from two AP and 
      two BP of CML patients with pseudodiploid karyotypes by conventional cytogenetics 
      were examined by FISH for trisomy 8 and i(17q). These SCA are major secondary 
      chromosomal changes seen in BP of CML patients. Results were considered positive 
      if more than 2.4% of cells had +8 and >6.25% for i(17q) by FISH. Four out of four 
      patients were positive for +8. These results indicate that FISH techniques are 
      valuable in the determination of SCA in CML, which were t(9;22)(q34;q11.2) 
      positive as the sole cytogenetic abnormality with standard G-banding karyotyping 
      and can be helpful for the early diagnosis of CML progression.
FAU - Wang, Ying
AU  - Wang Y
AD  - Cytogenetic Technology, School of Health Sciences, University of Texas M.D. 
      Anderson Cancer Center, Unit 350, 1515 Holcombe Boulevard, Houston, TX 
      77030-4009, USA.
FAU - Hopwood, Vicki L
AU  - Hopwood VL
FAU - Hu, Peter
AU  - Hu P
FAU - Lennon, Alan
AU  - Lennon A
FAU - Osterberger, Julie
AU  - Osterberger J
FAU - Glassman, Armand
AU  - Glassman A
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Blast Crisis/genetics/pathology
MH  - Bone Marrow/pathology
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human, Pair 17/genetics/ultrastructure
MH  - Chromosomes, Human, Pair 8/genetics
MH  - Disease Progression
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*genetics/pathology
MH  - Leukemia, Myeloid, Accelerated Phase/genetics/pathology
MH  - Male
MH  - Prognosis
MH  - Trisomy
EDAT- 2004/08/25 05:00
MHDA- 2004/10/01 05:00
CRDT- 2004/08/25 05:00
PHST- 2003/07/29 00:00 [received]
PHST- 2003/12/15 00:00 [revised]
PHST- 2003/12/17 00:00 [accepted]
PHST- 2004/08/25 05:00 [pubmed]
PHST- 2004/10/01 05:00 [medline]
PHST- 2004/08/25 05:00 [entrez]
AID - S0165460803005399 [pii]
AID - 10.1016/j.cancergencyto.2003.12.013 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2004 Aug;153(1):53-6. doi: 
      10.1016/j.cancergencyto.2003.12.013.