PMID- 15325639
OWN - NLM
STAT- MEDLINE
DCOM- 20041217
LR  - 20181130
IS  - 1063-4584 (Print)
IS  - 1063-4584 (Linking)
VI  - 12
IP  - 9
DP  - 2004 Sep
TI  - Intermittent hydrostatic pressure inhibits matrix metalloproteinase and 
      pro-inflammatory mediator release from human osteoarthritic chondrocytes in 
      vitro.
PG  - 729-35
AB  - OBJECTIVE: This study tested the hypothesis that intermittent hydrostatic 
      pressure applied to human osteoarthritic chondrocytes modulates matrix 
      metalloproteinase and pro-inflammatory mediator release in vitro. DESIGN: Human 
      osteoarthritic articular chondrocytes were isolated and cultured as primary 
      high-density monolayers. For testing, chondrocyte cultures were transferred to 
      serum-free medium and maintained without loading or with exposure to intermittent 
      hydrostatic pressure (IHP) at 10 MPa at a frequency of 1 Hz for periods of 6, 12 
      and 24 h. Levels of matrix metalloproteinase-2, -9 (MMP-2, -9), tissue inhibitor 
      of metalloproteinase-1 (TIMP-1), and the pro-inflammatory mediators, 
      interleukin-6 (IL-6) and monocyte chemoattractant protein-1 (MCP-1), released 
      into the culture medium were assessed by ELISA. Matrix metalloproteinase activity 
      was confirmed by zymographic analysis. RESULTS: In the absence of IHP, levels of 
      MMP-2, TIMP-1, IL-6, and MCP-1 in the chondrocyte culture medium increased in a 
      time-dependent manner. Application of IHP decreased MMP-2 levels at all time 
      periods tested, relative to unloaded control cultures maintained for the same 
      time periods. Although 84/82 kDa bands were faintly detectable by zymography, 
      MMP-9 levels were not quantifiable in medium from loaded or unloaded cultures by 
      ELISA. TIMP-1 levels were not altered in response to IHP at any time period 
      tested. IL-6 and MCP-1 levels decreased in cultures exposed to IHP at 12 and 24 
      h, relative to unloaded control cultures maintained for the same time periods. 
      CONCLUSION: IHP decreased release of MMP-2, IL-6 and MCP-1 by osteoarthritic 
      chondrocytes in vitro suggesting that pressure influences cartilage stability by 
      modulating chondrocyte expression of these degradative and pro-inflammatory 
      proteins in vivo.
FAU - Trindade, Michael C D
AU  - Trindade MC
AD  - Orthopaedic Research Laboratory, Stanford University School of Medicine, 
      Stanford, CA 94305-5341, USA.
FAU - Shida, Jun-ichi
AU  - Shida J
FAU - Ikenoue, Takashi
AU  - Ikenoue T
FAU - Lee, Mel S
AU  - Lee MS
FAU - Lin, Eric Y
AU  - Lin EY
FAU - Yaszay, Burt
AU  - Yaszay B
FAU - Yerby, Scott
AU  - Yerby S
FAU - Goodman, Stuart B
AU  - Goodman SB
FAU - Schurman, David J
AU  - Schurman DJ
FAU - Smith, R Lane
AU  - Smith RL
LA  - eng
GR  - AR 45788/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Osteoarthritis Cartilage
JT  - Osteoarthritis and cartilage
JID - 9305697
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-6)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
SB  - IM
MH  - Cartilage, Articular/*enzymology/immunology
MH  - Cells, Cultured
MH  - Chemokine CCL2/analysis/metabolism
MH  - Chondrocytes/immunology/*metabolism
MH  - Cytokines/*metabolism
MH  - Humans
MH  - Hydrostatic Pressure
MH  - Interleukin-6/analysis/metabolism
MH  - Matrix Metalloproteinases/analysis/*metabolism
MH  - Osteoarthritis/*enzymology/immunology
MH  - Statistics, Nonparametric
EDAT- 2004/08/25 05:00
MHDA- 2004/12/18 09:00
CRDT- 2004/08/25 05:00
PHST- 2003/08/07 00:00 [received]
PHST- 2004/05/14 00:00 [accepted]
PHST- 2004/08/25 05:00 [pubmed]
PHST- 2004/12/18 09:00 [medline]
PHST- 2004/08/25 05:00 [entrez]
AID - S1063458404000937 [pii]
AID - 10.1016/j.joca.2004.05.008 [doi]
PST - ppublish
SO  - Osteoarthritis Cartilage. 2004 Sep;12(9):729-35. doi: 10.1016/j.joca.2004.05.008.