PMID- 15326386
OWN - NLM
STAT- MEDLINE
DCOM- 20050524
LR  - 20200930
IS  - 1538-4047 (Print)
IS  - 1538-4047 (Linking)
VI  - 3
IP  - 9
DP  - 2004 Sep
TI  - Digital HLA allelotyping.
PG  - 899-902
AB  - Stem cell transplantation is a potentially curative therapy for a number of 
      neoplastic disorders. Successful transplantation of bone marrow, and other 
      sources of stem cells, depends upon the extent to which human leukocyte antigen 
      (HLA) alleles in donor and recipient are matched. HLA incompatibility can result 
      in cytotoxic T-cell mediated graft failure or graft versus host disease. It has 
      been shown that accurate matching between donor and recipient minimizes both of 
      these outcomes and results in improved patient survival. Therefore, methods for 
      high resolution HLA analysis have substantial clinical impact. Heterozygosity at 
      the HLA loci interferes with current methods of DNA sequence-based HLA typing. We 
      present a novel method for HLA analysis, which we term Digital HLA Allelotyping, 
      or dig-HLA. This method employs a strategy in which the expressed HLA alleles are 
      randomly sorted and individually identified.
FAU - Topaloglu, Ozlem
AU  - Topaloglu O
AD  - Department of Radiation Oncology and Molecular Radiation Sciences, The Sidney 
      Kimmel Comprehensive Cancer Center, The Johns Hopkins School of Medicine, 
      Baltimore, Maryland 21231, USA.
FAU - Civin, Curt I
AU  - Civin CI
FAU - Bunz, Fred
AU  - Bunz F
LA  - eng
GR  - P30 CA06973/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20040915
PL  - United States
TA  - Cancer Biol Ther
JT  - Cancer biology & therapy
JID - 101137842
RN  - 0 (DNA, Complementary)
RN  - 0 (Histocompatibility Antigens)
SB  - IM
MH  - Alleles
MH  - DNA, Complementary
MH  - Histocompatibility Antigens/*analysis
MH  - Histocompatibility Testing/*methods
MH  - Humans
MH  - Polymerase Chain Reaction
MH  - Signal Processing, Computer-Assisted
MH  - *Stem Cell Transplantation
EDAT- 2004/08/25 05:00
MHDA- 2005/05/25 09:00
CRDT- 2004/08/25 05:00
PHST- 2004/08/25 05:00 [pubmed]
PHST- 2005/05/25 09:00 [medline]
PHST- 2004/08/25 05:00 [entrez]
AID - 1083 [pii]
AID - 10.4161/cbt.3.9.1083 [doi]
PST - ppublish
SO  - Cancer Biol Ther. 2004 Sep;3(9):899-902. doi: 10.4161/cbt.3.9.1083. Epub 2004 Sep 
      15.