PMID- 15327028
OWN - NLM
STAT- MEDLINE
DCOM- 20050208
LR  - 20170214
IS  - 1352-4585 (Print)
IS  - 1352-4585 (Linking)
VI  - 10
IP  - 4
DP  - 2004 Aug
TI  - Human herpesvirus 6 infects the central nervous system of multiple sclerosis 
      patients in the early stages of the disease.
PG  - 348-54
AB  - The presence and the replicative state of human herpesvirus 6 (HHV-6) were 
      evaluated in clinical samples from multiple sclerosis (MS) patients at the first 
      time of MS diagnosis. HHV-6 variant B was present in peripheral blood mononuclear 
      cells of 5/32 (15%) patients, but persisted with a latent infection. Viral 
      sequences were present also in cerebrospinal fluid (CSF), both free in the liquid 
      (7/32, 22%) and latent in the cellular fraction (3/32, 9%), as shown by analysis 
      of viral transcription. In these cases, variant A was detected. HHV-6 DNA 
      sequences present in the CSF were associated to mature viral particles. In fact, 
      in vitro infectious assays of CSF showed the presence of replication-competent 
      virions. These results show that about 20% of MS patients have active foci of 
      HHV-6 variant A infection in the early stages of the disease and suggest that 
      viral replication takes place within the central nervous system.
FAU - Rotola, A
AU  - Rotola A
AD  - Section of Microbiology, Department of Experimental and Diagnostic Medicine, 
      University of Ferrara, Italy.
FAU - Merlotti, I
AU  - Merlotti I
FAU - Caniatti, L
AU  - Caniatti L
FAU - Caselli, E
AU  - Caselli E
FAU - Granieri, E
AU  - Granieri E
FAU - Tola, M R
AU  - Tola MR
FAU - Di Luca, D
AU  - Di Luca D
FAU - Cassai, E
AU  - Cassai E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Mult Scler
JT  - Multiple sclerosis (Houndmills, Basingstoke, England)
JID - 9509185
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Central Nervous System Diseases/blood/cerebrospinal fluid/*complications
MH  - Cerebrospinal Fluid/virology
MH  - DNA, Viral/cerebrospinal fluid
MH  - Enzyme-Linked Immunosorbent Assay
MH  - *Herpesvirus 6, Human/genetics/physiology
MH  - Humans
MH  - Multiple Sclerosis/blood/cerebrospinal fluid/*complications
MH  - Polymerase Chain Reaction
MH  - Prevalence
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Roseolovirus Infections/*complications/epidemiology
MH  - Virion/physiology
MH  - Virus Latency
MH  - Virus Replication
EDAT- 2004/08/26 05:00
MHDA- 2005/02/09 09:00
CRDT- 2004/08/26 05:00
PHST- 2004/08/26 05:00 [pubmed]
PHST- 2005/02/09 09:00 [medline]
PHST- 2004/08/26 05:00 [entrez]
AID - 10.1191/1352458504ms1045oa [doi]
PST - ppublish
SO  - Mult Scler. 2004 Aug;10(4):348-54. doi: 10.1191/1352458504ms1045oa.