PMID- 15327029
OWN - NLM
STAT- MEDLINE
DCOM- 20050208
LR  - 20170214
IS  - 1352-4585 (Print)
IS  - 1352-4585 (Linking)
VI  - 10
IP  - 4
DP  - 2004 Aug
TI  - Distribution and quantification of human herpesvirus 6 in multiple sclerosis and 
      control brains.
PG  - 355-9
AB  - Multiple sclerosis (MS) is thought to be precipitated by environmental factors, 
      potentially including viruses, in genetically susceptible individuals and 
      recently human herpesvirus 6 (HHV-6) has been associated with the disease. We 
      have analysed post mortem brain for the presence, variant type and quantity of 
      HHV-6 by PCR. A total of 124 samples from seven anatomically defined regions of 
      brain were tested from MS cases and controls. HHV-6 DNA was detected in 41% and 
      44% of MS case and control samples. The median viral loads were 11 and 9 genome 
      copies/microg DNA in cases and controls respectively and the viral load was not 
      increased in lesions. Except in one instance, the HHV-6 DNA detected was variant 
      B. There was no apparent difference in the distribution of HHV-6 DNA in the 
      brains of MS cases versus controls, nor between normal appearing and lesional 
      tissue in MS cases. Periventricular regions of the brain were more frequently 
      positive for HHV-6 DNA in both MS cases and controls, although this difference 
      was not statistically significant. These studies confirm the neurotropism of 
      HHV-6 but do not demonstrate differences in the distribution, variant type or 
      quantity of HHV-6 in brains from patients with MS versus controls.
FAU - Tuke, P W
AU  - Tuke PW
AD  - Centre for Virology, Department of Infection, Royal Free and University College 
      Medical School of UCL, Hampstead Site, Rowland Hill Street, London NW3 2PF, UK.
FAU - Hawke, S
AU  - Hawke S
FAU - Griffiths, P D
AU  - Griffiths PD
FAU - Clark, D A
AU  - Clark DA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Mult Scler
JT  - Multiple sclerosis (Houndmills, Basingstoke, England)
JID - 9509185
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Brain/metabolism/pathology/*virology
MH  - Case-Control Studies
MH  - DNA, Viral/metabolism
MH  - Female
MH  - Gene Dosage
MH  - Herpesvirus 6, Human/genetics/*isolation & purification
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Multiple Sclerosis/metabolism/pathology/*virology
MH  - Polymerase Chain Reaction
MH  - Tissue Distribution
MH  - Viral Load
EDAT- 2004/08/26 05:00
MHDA- 2005/02/09 09:00
CRDT- 2004/08/26 05:00
PHST- 2004/08/26 05:00 [pubmed]
PHST- 2005/02/09 09:00 [medline]
PHST- 2004/08/26 05:00 [entrez]
AID - 10.1191/1352458504ms1057oa [doi]
PST - ppublish
SO  - Mult Scler. 2004 Aug;10(4):355-9. doi: 10.1191/1352458504ms1057oa.