PMID- 15328149
OWN - NLM
STAT- MEDLINE
DCOM- 20050324
LR  - 20210206
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 104
IP  - 13
DP  - 2004 Dec 15
TI  - Dynamic regulation of IL-7 receptor expression is required for normal 
      thymopoiesis.
PG  - 4165-72
AB  - Interleukin-7 receptor (IL-7R) levels are tightly controlled during ontogeny: 
      high on double-negative (DN) cells, absent on double-positive (DP) cells, and 
      high once again on thymocytes undergoing positive selection. To determine if loss 
      of IL-7-mediated survival signals in DP cells is necessary for normal 
      antigen-specific selection, we created T-lineage-specific IL-7R alpha chain 
      (IL-7Ralpha) transgenic (Tg) mice in which IL-7R is expressed throughout 
      ontogeny. There was no effect of the IL-7Ralpha Tg on negative selection. 
      Surprisingly, however, although the thymi of IL-7Ralpha Tg mice were comparable 
      at birth, there was a decrease in thymocyte number as the mice aged. This was 
      found to be due to competition between DN and IL-7R-expressing DP cells for 
      endogenous IL-7, which resulted in decreased levels of Bcl-2 in DN cells, 
      increased DN apoptosis, and decreased DN cell number. Therefore, the 
      down-regulation of IL-7R on DP cells is an "altruistic" act required for 
      maintaining an adequate supply of local IL-7 for DN cells.
FAU - Munitic, Ivana
AU  - Munitic I
AD  - Laboratory of Immune Cell Biology, National Cancer Institute, National Institutes 
      of Health, Bethesda, MD 20892, USA.
FAU - Williams, Joy A
AU  - Williams JA
FAU - Yang, Yili
AU  - Yang Y
FAU - Dong, Bei
AU  - Dong B
FAU - Lucas, Philip J
AU  - Lucas PJ
FAU - El Kassar, Nahed
AU  - El Kassar N
FAU - Gress, Ronald E
AU  - Gress RE
FAU - Ashwell, Jonathan D
AU  - Ashwell JD
LA  - eng
PT  - Journal Article
DEP - 20040824
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (DNA, Complementary)
RN  - 0 (Interleukin-7)
RN  - 0 (Receptors, Interleukin-7)
RN  - 0 (interleukin-7 receptor, alpha chain)
SB  - IM
MH  - Animals
MH  - Apoptosis/immunology
MH  - DNA, Complementary/genetics
MH  - Gene Expression Regulation/immunology
MH  - In Situ Nick-End Labeling
MH  - Interleukin-7/immunology
MH  - Lymphopoiesis/genetics/*immunology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Receptors, Interleukin-7/*genetics/*immunology
MH  - Selection, Genetic
MH  - T-Lymphocytes/*immunology
MH  - Thymus Gland/immunology
EDAT- 2004/08/26 05:00
MHDA- 2005/03/25 09:00
CRDT- 2004/08/26 05:00
PHST- 2004/08/26 05:00 [pubmed]
PHST- 2005/03/25 09:00 [medline]
PHST- 2004/08/26 05:00 [entrez]
AID - S0006-4971(20)49062-7 [pii]
AID - 10.1182/blood-2004-06-2484 [doi]
PST - ppublish
SO  - Blood. 2004 Dec 15;104(13):4165-72. doi: 10.1182/blood-2004-06-2484. Epub 2004 
      Aug 24.