PMID- 15328157
OWN - NLM
STAT- MEDLINE
DCOM- 20050324
LR  - 20220317
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 104
IP  - 13
DP  - 2004 Dec 15
TI  - CD157 is an important mediator of neutrophil adhesion and migration.
PG  - 4269-78
AB  - CD157, a glycosylphosphatidylinositol (GPI)-anchored protein encoded by a member 
      of the CD38 NADase/ADP-ribosyl cyclase gene family, is expressed on the surface 
      of most human circulating neutrophils. This work demonstrates that CD157 is a 
      receptor that induces reorganization of the cytoskeleton and significant changes 
      in cell shape, and that signals mediated by CD157 act through modulation of 
      cytosolic Ca(2+) concentration. These signals are independent of the products of 
      CD157's enzymatic activities (ie, cyclic adenosine diphosphate [ADP]-ribose and 
      ADP-ribose). Indeed, the enzymatic activities of CD157 in circulating neutrophils 
      as well as in dimethyl sulfoxide (DMSO)-differentiated (CD157(+)/CD38(-)) HL-60 
      cells, are hardly detectable. This work also shows that the receptorial activity 
      relies on cross-talk between CD157 and beta(2) integrin. CD157 localizes in 
      GM1-enriched lipid rafts and, upon activation, it migrates to the uropod, a 
      structure specialized in motility and adhesive functions. Indeed, CD157 is 
      involved in adhesion to extracellular matrix proteins and in chemotaxis induced 
      in vitro by formyl-methionyl-leucyl-phenylalanine (fMLP). These findings were 
      consistent with the results obtained in neutrophils from patients with paroxysmal 
      nocturnal hemoglobinuria (PNH), in which CD157 is deficient. These neutrophils 
      showed constant defects in adhesion and migration. Our data attribute specific 
      and crucial roles to CD157 in the regulation of innate immunity during 
      inflammation.
FAU - Funaro, Ada
AU  - Funaro A
AD  - Laboratory of Immunogenetics, Department of Genetics, Biology and Biochemistry, 
      University of Torino, Via Santena 19, 10126 Torino, Italy. ada.funaro@unito.it
FAU - Ortolan, Erika
AU  - Ortolan E
FAU - Ferranti, Bruna
AU  - Ferranti B
FAU - Gargiulo, Lucia
AU  - Gargiulo L
FAU - Notaro, Rosario
AU  - Notaro R
FAU - Luzzatto, Lucio
AU  - Luzzatto L
FAU - Malavasi, Fabio
AU  - Malavasi F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20040824
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antigens, CD)
RN  - 0 (GPI-Linked Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (phosphatidylinositol glycan-class A protein)
RN  - EC 3.2.2.5 (ADP-ribosyl Cyclase)
RN  - EC 3.2.2.5 (ADP-ribosyl cyclase 2)
SB  - IM
MH  - ADP-ribosyl Cyclase/genetics/*physiology
MH  - Antigens, CD/genetics/*physiology
MH  - Cell Adhesion/*physiology
MH  - Cell Division/physiology
MH  - Chemotaxis, Leukocyte/*physiology
MH  - GPI-Linked Proteins
MH  - Humans
MH  - Membrane Proteins/*blood
MH  - Multigene Family
MH  - Neutrophils/*physiology
MH  - Reference Values
EDAT- 2004/08/26 05:00
MHDA- 2005/03/25 09:00
CRDT- 2004/08/26 05:00
PHST- 2004/08/26 05:00 [pubmed]
PHST- 2005/03/25 09:00 [medline]
PHST- 2004/08/26 05:00 [entrez]
AID - S0006-4971(20)49075-5 [pii]
AID - 10.1182/blood-2004-06-2129 [doi]
PST - ppublish
SO  - Blood. 2004 Dec 15;104(13):4269-78. doi: 10.1182/blood-2004-06-2129. Epub 2004 
      Aug 24.