PMID- 15329478
OWN - NLM
STAT- MEDLINE
DCOM- 20050208
LR  - 20190607
IS  - 0916-8818 (Print)
IS  - 0916-8818 (Linking)
VI  - 50
IP  - 4
DP  - 2004 Aug
TI  - Assessment of bovine X- and Y-bearing spermatozoa in fractions by discontinuous 
      percoll gradients with rapid fluorescence in situ hybridization.
PG  - 463-9
AB  - This study was designed to apply the method of discontinuous Percoll gradients 
      for sex preselection in bovine semen by using a current developed molecular 
      technique, fluorescence in situ hybridization (FISH). In addition, we attempted 
      to amplify the level of enrichment of X- or Y-bearing spermatozoa by treating for 
      activating sperm motility performance with 10 mM caffeine. Bovine spermatozoa 
      were fractionated on Percoll gradients into two major subpopulations of motile 
      spermatozoa (bottom fraction) and weak motile spermatozoa (top fraction). The 
      percentage of Y-bearing spermatozoa in the top fraction (52.9%) slightly exceeded 
      and that in the bottom fraction (44.3%) decreased significantly (P<0.001) 
      compared with the theoretical ratio (50:50). Washing sperm with BO medium 
      affected a deviation between the two sex populations, whereas semen activated 
      with caffeine showed no difference in the percentage of X- and Y-bearing 
      spermatozoa in both fractions compared with the theoretical ratio (50:50). These 
      results show that the proportion of X- and Y-bearing bovine spermatozoa can 
      deviate after discontinuous Percoll gradients, although the proportion of X- and 
      Y-bearing bovine spermatozoa was affected by sperm motility of the sample 
      applied.
FAU - Kobayashi, Jin
AU  - Kobayashi J
AD  - Miyagi Agricultural College, Sendai, Japan. kobayashi@miyanou.ac.jp
FAU - Oguro, Hitoshi
AU  - Oguro H
FAU - Uchida, Hiroshi
AU  - Uchida H
FAU - Kohsaka, Tetsuya
AU  - Kohsaka T
FAU - Sasada, Hiroshi
AU  - Sasada H
FAU - Sato, Eimei
AU  - Sato E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - J Reprod Dev
JT  - The Journal of reproduction and development
JID - 9438792
RN  - 65455-52-9 (Percoll)
RN  - 7631-86-9 (Silicon Dioxide)
RN  - FZ989GH94E (Povidone)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cell Separation/*methods
MH  - Fertilization in Vitro/veterinary
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Povidone
MH  - Sex Preselection/*methods/*veterinary
MH  - Silicon Dioxide
MH  - Spermatozoa/*cytology
MH  - X Chromosome
MH  - Y Chromosome
EDAT- 2004/08/27 05:00
MHDA- 2005/02/09 09:00
CRDT- 2004/08/27 05:00
PHST- 2004/08/27 05:00 [pubmed]
PHST- 2005/02/09 09:00 [medline]
PHST- 2004/08/27 05:00 [entrez]
AID - JST.JSTAGE/jrd/50.463 [pii]
AID - 10.1262/jrd.50.463 [doi]
PST - ppublish
SO  - J Reprod Dev. 2004 Aug;50(4):463-9. doi: 10.1262/jrd.50.463.