PMID- 15330094
OWN - NLM
STAT- MEDLINE
DCOM- 20050228
LR  - 20191210
IS  - 0021-9673 (Print)
IS  - 0021-9673 (Linking)
VI  - 1043
IP  - 2
DP  - 2004 Jul 23
TI  - Analysis of mono- and disaccharides in milk-based formulae by high-performance 
      liquid chromatography with refractive index detection.
PG  - 211-5
AB  - A simple and reproducible method for the qualitative and quantitative analysis of 
      free mono- and disaccharides (fructose, glucose, galactose, sucrose, lactulose 
      and lactose) in milk-based formulae by high-performance liquid chromatography 
      (HPLC) with refractive index (RI) detection was developed and validated. The 
      method showed good linearity with determination coefficients exceeding 0.99. The 
      limits of detection (DL) in these sugars were 0.17, 0.13, 0.06, 0.16, 0.05 and 
      0.25 mg/ml, respectively; and the limits of quantification (QL), 0.27, 0.24, 
      0.20, 0.26, 0.22 and 0.38 mg/ml. The relative standard deviations (R.S.D.s) for 
      repeatability in fructose, sucrose, lactulose and lactose were 0.78, 0.99, 2.91 
      and 0.46 and the R.S.D.s for reproducibility were 4.8, 6.15, 7.04 and 2.49, 
      respectively. Recoveries in all sugars were between 93 and 113%.
FAU - Chavez-Servin, Jorge L
AU  - Chavez-Servin JL
AD  - Dept Nutricio i Bromatologia, Centre de Referencia en Tecnologia dels Aliments, 
      Facultat de Farmacia, Universitat de Barcelona, Avda Joan XXIII s/n, 08028 
      Barcelona, Spain.
FAU - Castellote, Ana I
AU  - Castellote AI
FAU - Lopez-Sabater, M Carmen
AU  - Lopez-Sabater MC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 30237-26-4 (Fructose)
RN  - 4618-18-2 (Lactulose)
RN  - 57-50-1 (Sucrose)
RN  - J2B2A4N98G (Lactose)
SB  - IM
MH  - Animals
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Fructose/*analysis
MH  - Lactose/*analysis
MH  - Lactulose/*analysis
MH  - Milk/*chemistry
MH  - Refractometry/*methods
MH  - Reproducibility of Results
MH  - Sucrose/*analysis
EDAT- 2004/08/28 05:00
MHDA- 2005/03/01 09:00
CRDT- 2004/08/28 05:00
PHST- 2004/08/28 05:00 [pubmed]
PHST- 2005/03/01 09:00 [medline]
PHST- 2004/08/28 05:00 [entrez]
AID - S0021-9673(04)00880-5 [pii]
AID - 10.1016/j.chroma.2004.06.002 [doi]
PST - ppublish
SO  - J Chromatogr A. 2004 Jul 23;1043(2):211-5. doi: 10.1016/j.chroma.2004.06.002.