PMID- 15330899
OWN - NLM
STAT- MEDLINE
DCOM- 20041007
LR  - 20220318
IS  - 0002-9270 (Print)
IS  - 0002-9270 (Linking)
VI  - 99
IP  - 9
DP  - 2004 Sep
TI  - Significance of macrophage chemoattractant protein-1 expression and macrophage 
      infiltration in squamous cell carcinoma of the esophagus.
PG  - 1667-74
AB  - OBJECTIVES: Macrophage chemoattractant protein-1 (MCP-1) is a chemokine-inducing 
      infiltration of macrophages, which can play several roles in tumor growth and 
      metastasis. We have attempted to clarify the relationship between MCP-1 
      expression and macrophage infiltration in esophageal squamous cell carcinoma 
      (SCC). METHODS: Paraffin-embedded sections of tissue samples taken from 56 
      patients with esophageal SCC after curative surgery were immunohistochemically 
      stained for MCP-1, CC chemokine receptor 2 (CCR-2), and thymidine phosphorylase 
      (TP). Macrophage recruitment in SCC was evaluated by monocytic count based on 
      CD68 immunostaining. Microvessels immunostained for Factor VIII-related antigen 
      were counted in SCC, and microvessel density (MVD) was determined. Ki-67 labeling 
      index was calculated based on Ki-67 immunostaining, and an apoptotic index was 
      calculated based on the terminal deoxynucleotidyl transferase-mediated 
      deoxyuridine triphosphate biotin nick end labeling. RESULTS: MCP-1 was expressed 
      in cancer cells of 31 SCC (55.4%) and in stromal cells mainly identified as 
      macrophages of 16 SCC (28.6%). CCR-2 was expressed in stromal cells of all SCC 
      and in vascular endothelial cells of 15 SCC (26.8%). There was a significant 
      correlation between the expression of MCP-1 in cancer cells and of CCR-2 in 
      stromal cells. TP was expressed in stromal cells in 76.7% of the SCC. Monocytic 
      count, MVD, and Ki-67 LI in SCC with MCP-1 expression in cancer cells were higher 
      than that without, and apoptotic index in SCC with MCP-1 expression in cancer 
      cells were lower than that without. Furthermore, the monocytic count was 
      positively correlated with MVD, while it was inversely correlated with apoptotic 
      index. Clinicopathologically, MCP-1 expression in cancer cells was correlated 
      with venous invasion, distant metastasis, and lymph node metastasis. Monocytic 
      count in SCC with venous invasion, distant metastasis, or lymph node metastasis 
      was higher than that without them. Five-year survival rate in the patients with 
      high monocytic count or MCP-1 expression was worse than that with a low monocytic 
      count or without MCP-1 expression. CONCLUSIONS: These results suggest that MCP-1 
      expression and macrophage infiltration is associated with angiogenic promotion in 
      esophageal SCC. MCP-1 expression may be interactively associated with macrophage 
      infiltration in esophageal SCC; MCP-1 may play an important role in tumor 
      angiogenesis through production of angiogenic factors, such as TP, by recruited 
      macrophages in esophageal SCC. Furthermore, CCR-2 expression in vascular 
      endothelial cells may participate partially in angiogenesis. 
      Clinicopathologically, esophageal SCC patients with MCP-1 expression have no 
      favorable prognosis.
FAU - Koide, Naohiko
AU  - Koide N
AD  - Department of Surgery, Shinshu University School of Medicine, 3-1-1 Asahi, 
      Matsumoto 390-8621, Japan.
FAU - Nishio, Akihito
AU  - Nishio A
FAU - Sato, Toshiyuki
AU  - Sato T
FAU - Sugiyama, Atsushi
AU  - Sugiyama A
FAU - Miyagawa, Shin-ichi
AU  - Miyagawa S
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Am J Gastroenterol
JT  - The American journal of gastroenterology
JID - 0421030
RN  - 0 (Angiogenesis Inducing Agents)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Chemokine CCL2)
SB  - IM
MH  - Angiogenesis Inducing Agents/metabolism
MH  - Biomarkers, Tumor/*metabolism
MH  - Biopsy, Needle
MH  - Carcinoma, Squamous Cell/metabolism/*mortality/*pathology/surgery
MH  - Cell Movement
MH  - Chemokine CCL2/*metabolism
MH  - Cohort Studies
MH  - Culture Techniques
MH  - Esophageal Neoplasms/metabolism/*mortality/*pathology/surgery
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Nick-End Labeling
MH  - Japan/epidemiology
MH  - Macrophages/physiology
MH  - Male
MH  - Neoplasm Staging
MH  - Predictive Value of Tests
MH  - Probability
MH  - Prognosis
MH  - Proportional Hazards Models
MH  - Retrospective Studies
MH  - Risk Assessment
MH  - Statistics, Nonparametric
MH  - Survival Analysis
EDAT- 2004/08/28 05:00
MHDA- 2004/10/08 09:00
CRDT- 2004/08/28 05:00
PHST- 2004/08/28 05:00 [pubmed]
PHST- 2004/10/08 09:00 [medline]
PHST- 2004/08/28 05:00 [entrez]
AID - AJG30733 [pii]
AID - 10.1111/j.1572-0241.2004.30733.x [doi]
PST - ppublish
SO  - Am J Gastroenterol. 2004 Sep;99(9):1667-74. doi: 
      10.1111/j.1572-0241.2004.30733.x.