PMID- 15330900
OWN - NLM
STAT- MEDLINE
DCOM- 20041007
LR  - 20071114
IS  - 0002-9270 (Print)
IS  - 0002-9270 (Linking)
VI  - 99
IP  - 9
DP  - 2004 Sep
TI  - A comparison of routine cytology and fluorescence in situ hybridization for the 
      detection of malignant bile duct strictures.
PG  - 1675-81
AB  - BACKGROUND AND AIM: The aim of this study was to assess the relative 
      sensitivities and specificities of fluorescence in situ hybridization (FISH) and 
      routine cytology for the detection of malignancy in biliary tract strictures. 
      METHODS: Bile duct brushing and aspirate specimens were collected from 131 
      patients being evaluated for possible malignant bile duct strictures. Both 
      specimen types were assessed by FISH but only brushing specimens were assessed by 
      cytology. The FISH assay used a mixture of fluorescently-labeled probes to the 
      centromeres of chromosomes 3, 7, and 17 and chromosomal band 9p21 (Vysis 
      UroVysion) to identify cells having chromosomal abnormalities. A case was 
      considered positive for malignancy if five or more cells exhibited polysomy. 
      RESULTS: Sixty-six of the 131 patients had surgical pathologic and/or clinical 
      evidence of malignancy. Thirty-nine patients had cholangiocarcinoma, 19 had 
      pancreatic carcinoma, and 8 had other types of malignancy. The sensitivity of 
      cytology and FISH for the detection of malignancy in bile duct brushing specimens 
      in these patients was 15% and 34% (p < 0.01), respectively. The sensitivity of 
      FISH for the bile aspirate specimens was 23%, and the combined sensitivity of 
      FISH for aspirate and brushing specimens was 35%. The specificity of FISH and 
      cytology brushings were 91% and 98% (p= 0.06), respectively. CONCLUSIONS: FISH is 
      significantly more sensitive than and nearly as specific as conventional cytology 
      for the detection of malignant biliary strictures in biliary brushing specimens. 
      FISH may improve the clinical management of patients who are being evaluated for 
      malignancy in bile duct strictures.
FAU - Kipp, Benjamin R
AU  - Kipp BR
AD  - Department of Laboratory Medicine and Pathology, Mayo Clinic College of Medicine, 
      200 First Street SW, Rochester, MN 55905, USA.
FAU - Stadheim, Linda M
AU  - Stadheim LM
FAU - Halling, Shari A
AU  - Halling SA
FAU - Pochron, Nicole L
AU  - Pochron NL
FAU - Harmsen, Scott
AU  - Harmsen S
FAU - Nagorney, David M
AU  - Nagorney DM
FAU - Sebo, Thomas J
AU  - Sebo TJ
FAU - Therneau, Terry M
AU  - Therneau TM
FAU - Gores, Gregory J
AU  - Gores GJ
FAU - de Groen, Piet C
AU  - de Groen PC
FAU - Baron, Todd H
AU  - Baron TH
FAU - Levy, Michael J
AU  - Levy MJ
FAU - Halling, Kevin C
AU  - Halling KC
FAU - Roberts, Lewis R
AU  - Roberts LR
LA  - eng
GR  - CA 100882/CA/NCI NIH HHS/United States
GR  - CA 82862/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Gastroenterol
JT  - The American journal of gastroenterology
JID - 0421030
SB  - IM
CIN - Am J Gastroenterol. 2004 Sep;99(9):1682-3. PMID: 15330901
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Bile Duct Neoplasms/diagnosis/*pathology
MH  - Bile Ducts/*pathology
MH  - Biopsy, Needle
MH  - Cholangiopancreatography, Endoscopic Retrograde
MH  - Cohort Studies
MH  - Cytodiagnosis/methods
MH  - Diagnostic Tests, Routine
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Pancreatic Ducts/*pathology
MH  - Probability
MH  - Prospective Studies
MH  - Sensitivity and Specificity
EDAT- 2004/08/28 05:00
MHDA- 2004/10/08 09:00
CRDT- 2004/08/28 05:00
PHST- 2004/08/28 05:00 [pubmed]
PHST- 2004/10/08 09:00 [medline]
PHST- 2004/08/28 05:00 [entrez]
AID - AJG30281 [pii]
AID - 10.1111/j.1572-0241.2004.30281.x [doi]
PST - ppublish
SO  - Am J Gastroenterol. 2004 Sep;99(9):1675-81. doi: 
      10.1111/j.1572-0241.2004.30281.x.