PMID- 15331709
OWN - NLM
STAT- MEDLINE
DCOM- 20040928
LR  - 20181113
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 78
IP  - 18
DP  - 2004 Sep
TI  - Human immunodeficiency virus type 1 gp120 induces abnormal maturation and 
      functional alterations of dendritic cells: a novel mechanism for AIDS 
      pathogenesis.
PG  - 9763-72
AB  - Dendritic cells (DCs) play a crucial role in bridging innate and acquired immune 
      responses to pathogens. In human immunodeficiency virus type 1 (HIV-1) infection, 
      immature DCs (iDCs) are also main targets for HIV-1 at the mucosal level. In this 
      study, we evaluated the effects of HIV-1-DC interactions on the maturation and 
      functional activity of these cells. Exposure of human monocyte-derived iDCs to 
      either aldrithiol-2-inactivated HIV-1 or gp120 led to an upmodulation of 
      activation markers indicative of functional maturation. Despite their phenotype, 
      these cells retained antigen uptake capacity and showed an impaired ability to 
      secrete cytokines or chemokines and to induce T-cell proliferation. Although 
      gp120 did not interfere with DC differentiation, the capacity of these cells to 
      produce interleukin-12 (IL-12) upon maturation was markedly reduced. Likewise, 
      iDCs stimulated by classical maturation factors in the presence of gp120 lacked 
      allostimulatory capacity and did not produce IL-12, in spite of their phenotype 
      typical of activated DCs. Exogenous addition of IL-12 restores the 
      allostimulatory capacity of gp120-exposed DCs. The finding that gp120 induces 
      abnormal maturation of DCs linked to profound suppression of their activities 
      unravels a novel mechanism by which HIV can lead to immune dysfunction in AIDS 
      patients.
CI  - Copyright 2004 American Society for Microbiology
FAU - Fantuzzi, Laura
AU  - Fantuzzi L
AD  - Department of Cell Biology and Neurosciences, Istituto Superiore di Sanita, Viale 
      Regina Elena 299, 00161 Rome, Italy.
FAU - Purificato, Cristina
AU  - Purificato C
FAU - Donato, Karim
AU  - Donato K
FAU - Belardelli, Filippo
AU  - Belardelli F
FAU - Gessani, Sandra
AU  - Gessani S
LA  - eng
GR  - R21 AI054215/AI/NIAID NIH HHS/United States
GR  - 1 R21 AI054215-01/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (HIV Envelope Protein gp120)
RN  - 0 (Isoantigens)
RN  - 187348-17-0 (Interleukin-12)
SB  - IM
MH  - Acquired Immunodeficiency Syndrome/*etiology/immunology
MH  - Cell Differentiation
MH  - Dendritic Cells/immunology/*pathology/physiology/*virology
MH  - HIV Envelope Protein gp120/*physiology
MH  - HIV-1/*pathogenicity/physiology
MH  - Humans
MH  - In Vitro Techniques
MH  - Interleukin-12/biosynthesis
MH  - Isoantigens
MH  - Models, Biological
MH  - Phenotype
MH  - Virus Inactivation
PMC - PMC515003
EDAT- 2004/08/28 05:00
MHDA- 2004/09/29 05:00
PMCR- 2004/09/01
CRDT- 2004/08/28 05:00
PHST- 2004/08/28 05:00 [pubmed]
PHST- 2004/09/29 05:00 [medline]
PHST- 2004/08/28 05:00 [entrez]
PHST- 2004/09/01 00:00 [pmc-release]
AID - 78/18/9763 [pii]
AID - 0765-04 [pii]
AID - 10.1128/JVI.78.18.9763-9772.2004 [doi]
PST - ppublish
SO  - J Virol. 2004 Sep;78(18):9763-72. doi: 10.1128/JVI.78.18.9763-9772.2004.