PMID- 15333955
OWN - NLM
STAT- MEDLINE
DCOM- 20050225
LR  - 20071114
IS  - 0907-4449 (Print)
IS  - 0907-4449 (Linking)
VI  - 60
IP  - Pt 9
DP  - 2004 Sep
TI  - Structure of D-ribulose 5-phosphate 3-epimerase from Synechocystis to 1.6 A 
      resolution.
PG  - 1687-90
AB  - The crystal structure of D-ribulose 5-phosphate 3-epimerase (RPE) from the 
      cyanobacterium Synechocystis was determined by X-ray crystallography to 1.6 A 
      resolution. The enzyme, which catalyzes the epimerization of D-ribulose 
      5-phosphate and D-xylulose 5-phosphate, assembles as a hexamer of 
      (beta/alpha)(8)-barrels in the crystallographic asymmetric unit. The active site 
      is highly similar to those of two previously reported RPEs and provides further 
      evidence for essential catalytic roles for several active-site residues.
FAU - Wise, Eric L
AU  - Wise EL
AD  - Deparment of Biochemistry, University of Wisconsin, Madison, WI 53706, USA.
FAU - Akana, Julie
AU  - Akana J
FAU - Gerlt, John A
AU  - Gerlt JA
FAU - Rayment, Ivan
AU  - Rayment I
LA  - eng
GR  - GM-52594/GM/NIGMS NIH HHS/United States
GR  - GM-65155/GM/NIGMS NIH HHS/United States
GR  - GM08293/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20040826
PL  - United States
TA  - Acta Crystallogr D Biol Crystallogr
JT  - Acta crystallographica. Section D, Biological crystallography
JID - 9305878
RN  - EC 5.1.3.- (Carbohydrate Epimerases)
RN  - EC 5.1.3.1 (ribulosephosphate 3-epimerase)
SB  - IM
MH  - Binding Sites
MH  - Carbohydrate Epimerases/*chemistry/genetics
MH  - Cloning, Molecular
MH  - Crystallization
MH  - Crystallography, X-Ray
MH  - Cytosol/enzymology
MH  - Models, Molecular
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Synechocystis/*enzymology/genetics
EDAT- 2004/08/31 05:00
MHDA- 2005/02/26 09:00
CRDT- 2004/08/31 05:00
PHST- 2004/05/10 00:00 [received]
PHST- 2004/06/29 00:00 [accepted]
PHST- 2004/08/31 05:00 [pubmed]
PHST- 2005/02/26 09:00 [medline]
PHST- 2004/08/31 05:00 [entrez]
AID - S0907444904015896 [pii]
AID - 10.1107/S0907444904015896 [doi]
PST - ppublish
SO  - Acta Crystallogr D Biol Crystallogr. 2004 Sep;60(Pt 9):1687-90. doi: 
      10.1107/S0907444904015896. Epub 2004 Aug 26.