PMID- 15334550
OWN - NLM
STAT- MEDLINE
DCOM- 20041025
LR  - 20190816
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 41
IP  - 3
DP  - 2004 Nov
TI  - MLL translocations with concurrent 3' deletions: interpretation of FISH results.
PG  - 266-71
AB  - Rearrangements involving the MLL gene at 11q23 occur in a clinically relevant 
      subgroup of patients with acute lymphoblastic leukemia (ALL) at all ages, and 
      therefore their accurate identification at diagnosis is important. It has become 
      commonplace to screen ALL patients for rearrangements of MLL using a dual-color 
      fluorescence in situ hybridization (FISH) assay. We report on 12 ALL patients 
      with an unusual FISH result consisting of the following signal pattern: one 5' 
      green, no 3' red, and one/two fusion signals. This configuration is consistent 
      with a MLL translocation and simultaneous deletion of 3' MLL-a well-established 
      phenomenon-which has been interpreted as a positive result. G-banded and 
      complementary metaphase FISH analyses confirmed an 11q23/MLL translocation in 8 
      of the 12 cases, whereas in one case, the identification of a del(11)(q23) was 
      restricted to G-banded analysis only. In three cases, an MLL rearrangement was 
      excluded by extensive FISH analysis and/or Southern blotting. In conclusion, the 
      loss of the 3' MLL signal should not be assumed to be the result of a concurrent 
      translocation and deletion event, and such aberrant FISH signal patterns should 
      be investigated further by alternative methods for determining their MLL status.
CI  - Copyright 2004 Wiley-Liss, Inc.
FAU - Barber, Kerry E
AU  - Barber KE
AD  - Leukaemia Research Fund Cytogenetics Group, Cancer Sciences Division, University 
      of Southampton, Southampton, United Kingdom.
FAU - Ford, Anthony M
AU  - Ford AM
FAU - Harris, Rachel L
AU  - Harris RL
FAU - Harrison, Christine J
AU  - Harrison CJ
FAU - Moorman, Anthony V
AU  - Moorman AV
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (KMT2A protein, human)
RN  - 0 (Transcription Factors)
RN  - 149025-06-9 (Myeloid-Lymphoid Leukemia Protein)
RN  - EC 2.1.1.43 (Histone-Lysine N-Methyltransferase)
SB  - IM
MH  - Blotting, Southern
MH  - Child
MH  - Child, Preschool
MH  - Chromosome Banding
MH  - Chromosomes, Human, Pair 11
MH  - DNA-Binding Proteins/*genetics
MH  - Female
MH  - *Gene Deletion
MH  - Histone-Lysine N-Methyltransferase
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infant
MH  - Karyotyping
MH  - Male
MH  - Metaphase
MH  - Myeloid-Lymphoid Leukemia Protein
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*genetics
MH  - Proto-Oncogenes/*genetics
MH  - Statistics as Topic/methods
MH  - Transcription Factors/*genetics
MH  - *Translocation, Genetic
EDAT- 2004/08/31 05:00
MHDA- 2004/10/27 09:00
CRDT- 2004/08/31 05:00
PHST- 2004/08/31 05:00 [pubmed]
PHST- 2004/10/27 09:00 [medline]
PHST- 2004/08/31 05:00 [entrez]
AID - 10.1002/gcc.20082 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2004 Nov;41(3):266-71. doi: 10.1002/gcc.20082.