PMID- 15336377
OWN - NLM
STAT- MEDLINE
DCOM- 20050301
LR  - 20191210
IS  - 0731-7085 (Print)
IS  - 0731-7085 (Linking)
VI  - 35
IP  - 5
DP  - 2004 Sep 3
TI  - Simultaneous determination of serum flecainide and its metabolites by using high 
      performance liquid chromatography.
PG  - 1307-12
AB  - Simultaneous determination of serum flecainide and its oxidative metabolites was 
      carried out by using high performance liquid chromatography (HPLC) equipped with 
      conventional octadecylsilyl silica (ODS) column and fluorescence detector. 
      Flecainide and its metabolites, m-O-dealkylated flecainide (MODF) and 
      m-O-dealkylated lactam of flecainide (MODLF) in serum were extracted with ethyl 
      acetate. The recoveries of flecainide, MODF and MODLF were greater than 92, 93, 
      and 60% with the coefficient of variations (CVs) less than 3.2, 5.8, and 5.3%, 
      respectively. The calibration curves were linear at the concentration range of 
      50-1500 ng/mL for flecainide and 10-500 ng/mL for MODF and MODLF (r > 0.999). The 
      CVs for intra-day assay were 2.7-5.3% for flecainide, 3.0-4.2% for MODF, and 
      3.7-4.3% for MODLF, respectively. The CVs for inter-day assay were 7.0-8.4% for 
      flecainide, 3.3-6.7% for MODF, and 4.4-7.7% for MODLF, respectively. This assay 
      method can be used for assessing the metabolic ability of flecainide in the 
      patients with tachyarrhythmia.
FAU - Doki, Kosuke
AU  - Doki K
AD  - Department of Pharmaceutical Sciences, Institute of Clinical Medicine, University 
      of Tsukuba, Ten-nodai 1-1-1, Tsukuba, Ibaraki 305-8575, Japan.
FAU - Homma, Masato
AU  - Homma M
FAU - Kuga, Keisuke
AU  - Kuga K
FAU - Watanabe, Shigeyuki
AU  - Watanabe S
FAU - Yamaguchi, Iwao
AU  - Yamaguchi I
FAU - Kohda, Yukinao
AU  - Kohda Y
LA  - eng
PT  - Journal Article
PT  - Validation Study
PL  - England
TA  - J Pharm Biomed Anal
JT  - Journal of pharmaceutical and biomedical analysis
JID - 8309336
RN  - 0 (Anti-Arrhythmia Agents)
RN  - K94FTS1806 (Flecainide)
SB  - IM
MH  - Adult
MH  - Anti-Arrhythmia Agents/blood
MH  - Chromatography, High Pressure Liquid/instrumentation
MH  - Female
MH  - Flecainide/analogs & derivatives/*blood/pharmacokinetics
MH  - Humans
MH  - Male
MH  - Molecular Structure
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
EDAT- 2004/09/01 05:00
MHDA- 2005/03/02 09:00
CRDT- 2004/09/01 05:00
PHST- 2004/03/09 00:00 [received]
PHST- 2004/04/13 00:00 [revised]
PHST- 2004/04/19 00:00 [accepted]
PHST- 2004/09/01 05:00 [pubmed]
PHST- 2005/03/02 09:00 [medline]
PHST- 2004/09/01 05:00 [entrez]
AID - S0731708504001888 [pii]
AID - 10.1016/j.jpba.2004.04.009 [doi]
PST - ppublish
SO  - J Pharm Biomed Anal. 2004 Sep 3;35(5):1307-12. doi: 10.1016/j.jpba.2004.04.009.