PMID- 15336503
OWN - NLM
STAT- MEDLINE
DCOM- 20041012
LR  - 20061115
IS  - 0014-4835 (Print)
IS  - 0014-4835 (Linking)
VI  - 79
IP  - 3
DP  - 2004 Sep
TI  - Effects of matrix metalloproteinase-3 gene transfer by electroporation in 
      glaucoma filter surgery.
PG  - 405-10
AB  - To develop gene therapy that can be applied to glaucoma-filtering surgery, we 
      studied effects of transfection of matrix metalloproteinase-3 (MMP-3) cDNA into 
      rabbit conjunctiva by electroporation (EP) on changes of intraocular pressure 
      (IOP) and bleb formation after glaucoma filtering surgery. pTracer-CMV2 vector 
      containing MMP-3 cDNA was transfected into rabbit conjunctiva by EP and MMP-3 
      expression was studied by reverse transcription (RT)-PCR, zymography and western 
      blot analysis. Three days after the EP transfection of MMP-3 cDNA or vector alone 
      into rabbit conjunctiva, trabeculectomy was performed at the place of 
      transfection in the presence or absence of 0.04% mitomycin C (MMC). Then changes 
      in IOPs and bleb formation were compared with each other. Expression of MMP-3 was 
      detected in conjunctiva until 30 days after transfection by EP. Trabeculectomy 
      following MMP-3 transfection caused significantly longer survival of filtering 
      bleb and decreased levels of IOP in comparison with controls (trabeculectomy 
      alone or trabeculectomy following vector transfection), and these levels were 
      almost identical to those of trabeculectomy with MMC. The present study indicates 
      that EP is effective to transfect some genes that promote the filtering bleb 
      formation in glaucoma surgery, such as MMP-3 gene, and this may be potentially 
      applicable to glaucoma-filtering surgery in glaucoma patients.
FAU - Mamiya, Kazuhisa
AU  - Mamiya K
AD  - Department of Ophthalmology, Hirosaki University School of Medicine, 5 Zaifucho, 
      036-8562, Japan. ooguro@cc.hirosaki-u.ac.jp
FAU - Ohguro, Hiroshi
AU  - Ohguro H
FAU - Ohguro, Ikuyo
AU  - Ohguro I
FAU - Metoki, Tomomi
AU  - Metoki T
FAU - Ishikawa, Fotoshi
AU  - Ishikawa F
FAU - Yamazaki, Hitoshi
AU  - Yamazaki H
FAU - Takano, Yoshiko
AU  - Takano Y
FAU - Ito, Tadashi
AU  - Ito T
FAU - Nakazawa, Mitsuru
AU  - Nakazawa M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Exp Eye Res
JT  - Experimental eye research
JID - 0370707
RN  - 0 (DNA, Circular)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Animals
MH  - Blotting, Western/methods
MH  - Conjunctiva/physiology
MH  - DNA, Circular/genetics
MH  - Electrophoresis, Polyacrylamide Gel/methods
MH  - Electroporation/methods
MH  - Genetic Vectors/genetics
MH  - Glaucoma/*surgery
MH  - Humans
MH  - Intraocular Pressure/*genetics
MH  - Matrix Metalloproteinase 3/*genetics
MH  - Rabbits
MH  - Trabeculectomy/methods
MH  - Transfection/*methods
EDAT- 2004/09/01 05:00
MHDA- 2004/10/13 09:00
CRDT- 2004/09/01 05:00
PHST- 2004/02/01 00:00 [received]
PHST- 2004/06/15 00:00 [accepted]
PHST- 2004/09/01 05:00 [pubmed]
PHST- 2004/10/13 09:00 [medline]
PHST- 2004/09/01 05:00 [entrez]
AID - S0014-4835(04)00170-8 [pii]
AID - 10.1016/j.exer.2004.06.017 [doi]
PST - ppublish
SO  - Exp Eye Res. 2004 Sep;79(3):405-10. doi: 10.1016/j.exer.2004.06.017.