PMID- 15338084
OWN - NLM
STAT- MEDLINE
DCOM- 20050421
LR  - 20220409
IS  - 0300-5623 (Print)
IS  - 0300-5623 (Linking)
VI  - 32
IP  - 5
DP  - 2004 Oct
TI  - The effect of sustained and local administration of epidermal growth factor on 
      improving bilateral testicular tissue after torsion.
PG  - 323-31
AB  - Epidermal growth factor (EGF) modulates Leydig cell proliferation, 
      steroidogenesis, spermiogenesis, and Sertoli cell activity. It plays an important 
      role in repairing ischemia-reperfusion injury in different tissues. The aim of 
      this study was to evaluate the effects of sustained and local administration of 
      EGF on improving bilateral testicular tissue after torsion. A total of 57 Wistar 
      albino rats were used. For the EGF transport system, 1x2 cm gelatin films 
      containing 2 microg EGF were used. Torsion was created by rotating the right 
      testis 720 degrees in a clockwise direction for 4 h in all groups except the 
      control group. Then, in the torsion group, bilateral orchiectomy was performed. 
      After returning the torsioned ipsilateral testes to their normal state, the 
      bilateral testes were wrapped by 1x2 cm unloaded gelatin films in the gelatin (G7 
      and G21) groups and, by 2 microg EGF loaded gelatin films in the EGF 7 and EGF 21 
      groups. The testes were removed on the seventh and 21st days, respectively, for 
      biochemical and histological examination. Histologically, Johnsen's 
      spermatogenesis criteria and mean seminiferous tubule diameter (MSTD) 
      measurements were used. The EGF7 group did not show significant loss of Sertoli 
      cells, while in the G7 group the number of these cells decreased. The ipsilateral 
      ischemic testis of the EGF21 group showed Leydig cell hyperplasia, and the 
      contralateral non-ischemic testes in this group were similar to the control 
      group. In the G21 group, the bilateral testes showed Sertoli cell only syndrome 
      in some sections, and most of the cells were undergoing apoptosis. The mean 
      spermatogenesis scores and MSTD in the EGF7 and EGF21 groups were higher than in 
      the G7 and G21 groups ( P<0.05). Malondialdehyde levels were significantly lower 
      in the EGF groups than in the G groups ( P<0.05). Glutathione peroxidase (GSH-Px) 
      levels in the G21 group were significantly higher than in the EGF21 group. Our 
      study shows that local and sustained EGF release after testicular torsion 
      improves bilateral testicular injury. EGF administration may be a new treatment 
      choice for bilaterally injured testis after detorsion without removing the 
      twisted testis.
FAU - Uguralp, S
AU  - Uguralp S
AD  - Department of Pediatric Surgery, Inonu University, Turgut Ozal Medical Center, 
      Malatya, Turkey.
FAU - Bay Karabulut, A
AU  - Bay Karabulut A
FAU - Mizrak, B
AU  - Mizrak B
FAU - Kaymaz, F
AU  - Kaymaz F
FAU - Kiziltay, Aysel
AU  - Kiziltay A
FAU - Hasirci, N
AU  - Hasirci N
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20040828
PL  - Germany
TA  - Urol Res
JT  - Urological research
JID - 0364311
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Epidermal Growth Factor/administration & dosage/*metabolism/*pharmacology
MH  - Glutathione Peroxidase/drug effects/metabolism
MH  - Hyperplasia
MH  - Leydig Cells/drug effects/pathology
MH  - Male
MH  - Malondialdehyde/analysis/metabolism
MH  - Orchiectomy
MH  - Rats
MH  - Rats, Wistar
MH  - Reperfusion Injury/metabolism/pathology/physiopathology
MH  - Seminiferous Tubules/pathology
MH  - Sertoli Cells/drug effects/pathology
MH  - Spermatic Cord Torsion/pathology/*physiopathology
MH  - Spermatogenesis/drug effects/physiology
MH  - Testis/*drug effects/metabolism/*pathology
MH  - Time Factors
EDAT- 2004/09/01 05:00
MHDA- 2005/04/22 09:00
CRDT- 2004/09/01 05:00
PHST- 2003/09/24 00:00 [received]
PHST- 2004/03/08 00:00 [accepted]
PHST- 2004/09/01 05:00 [pubmed]
PHST- 2005/04/22 09:00 [medline]
PHST- 2004/09/01 05:00 [entrez]
AID - 10.1007/s00240-004-0418-6 [doi]
PST - ppublish
SO  - Urol Res. 2004 Oct;32(5):323-31. doi: 10.1007/s00240-004-0418-6. Epub 2004 Aug 
      28.