PMID- 15338196
OWN - NLM
STAT- MEDLINE
DCOM- 20050517
LR  - 20101118
IS  - 0939-5555 (Print)
IS  - 0939-5555 (Linking)
VI  - 84
IP  - 2
DP  - 2005 Feb
TI  - Altered erythrocyte membrane characteristics during anemia in childhood acute 
      lymphoblastic leukemia.
PG  - 76-84
AB  - Anemia is a prominent feature in children with acute lymphoblastic leukemia 
      (ALL). To investigate the erythrocyte features during anemia in these patients, 
      we studied the altered characters of these cells and oxidative stress imposed in 
      their serum. This investigation reveals that erythrocytes from ALL patients show 
      (1) increased membrane fluidity detected by fluorescence anisotropy studies, 
      increased osmotic fragility detected by hemolysis of erythrocytes in different 
      saline concentrations, and increased hydrophobicity as measured by binding with 
      8-anilino-1-naphthalenesulfonic acid, (2) enhanced (approximately threefold) 
      glycosylation and sialylation, monitored by digoxigenin enzyme assay, and (3) 
      expression of disease-specific 210, 105, 83, 54, and 28 kDa 9-O-acetyl 
      sialoglycoconjugates (9-O-AcSGs) demonstrated by Western blot analysis and 
      fluorescence-activated cell sorter (FACS) analysis studies using Achatinin-H with 
      specificity towards 9-O-AcSAalpha2-6GalNAc as the analytical probe. (4) In 
      addition, induced oxidative stress was observed in the sera of these children as 
      indicated by increased nitric oxide (approximately fourfold) and thiobarbituric 
      acid (TBA) reactive species (twofold) as detected by Griess reaction and TBA 
      assay, respectively. For all the experiments, erythrocytes from normal 
      individuals served as controls. Thus, the altered membrane characteristics 
      together with their exposure to induced oxidative stress in serum are found to be 
      a few features restricted to diseased erythrocytes. Taken together, our results 
      are suggestive of their interplay in the contribution to the observed anemia in 
      these patients, which may be exploited for better management of the disease.
FAU - Ghosh, Shyamasree
AU  - Ghosh S
AD  - Immunobiology Division, Indian Institute of Chemical Biology, 4 Raja S.C. Mullick 
      Road, Kolkata, 700032, India.
FAU - Bandyopadhyay, Suman
AU  - Bandyopadhyay S
FAU - Bhattacharya, Dilip K
AU  - Bhattacharya DK
FAU - Mandal, Chitra
AU  - Mandal C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20040825
PL  - Germany
TA  - Ann Hematol
JT  - Annals of hematology
JID - 9107334
RN  - 0 (Sialic Acids)
RN  - 55717-54-9 (9-O-acetyl-N-acetylneuraminic acid)
SB  - IM
MH  - Anemia/blood/*etiology
MH  - Case-Control Studies
MH  - Child
MH  - Child, Preschool
MH  - Erythrocyte Membrane/*pathology
MH  - Female
MH  - Glycosylation
MH  - Humans
MH  - Hydrophobic and Hydrophilic Interactions
MH  - Infant
MH  - Male
MH  - Membrane Fluidity
MH  - Osmotic Fragility
MH  - Oxidative Stress
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*blood/complications
MH  - Sialic Acids
EDAT- 2004/09/01 05:00
MHDA- 2005/05/18 09:00
CRDT- 2004/09/01 05:00
PHST- 2004/05/26 00:00 [received]
PHST- 2004/07/23 00:00 [accepted]
PHST- 2004/09/01 05:00 [pubmed]
PHST- 2005/05/18 09:00 [medline]
PHST- 2004/09/01 05:00 [entrez]
AID - 10.1007/s00277-004-0933-0 [doi]
PST - ppublish
SO  - Ann Hematol. 2005 Feb;84(2):76-84. doi: 10.1007/s00277-004-0933-0. Epub 2004 Aug 
      25.