PMID- 15338268
OWN - NLM
STAT- MEDLINE
DCOM- 20050308
LR  - 20151119
IS  - 0302-766X (Print)
IS  - 0302-766X (Linking)
VI  - 318
IP  - 2
DP  - 2004 Nov
TI  - Characterisation of neurons expressing calbindin immunoreactivity in the ileum of 
      the unweaned and mature sheep.
PG  - 289-303
AB  - We have identified the enteric neuron types expressing immunoreactivity for the 
      calcium-binding protein calbindin D28k (CALB) in cryostat sections and 
      whole-mount preparations of myenteric (MP) and submucosal (SMP) plexuses of sheep 
      ileum. We wished to determine whether CALB-IR in the sheep enteric nervous system 
      was expressed in Dogiel type II cells, as in guinea-pig and rat ileum, and could 
      therefore be used as a marker for intrinsic primary afferent neurons. The 
      neurochemical coding of CALB-containing myenteric and submucosal neurons in ileum 
      of unweaned lamb and mature sheep and its co-localisation with various neural 
      markers was studied immunohistochemically. An antiserum against neuronal nuclear 
      protein (NeuN) failed to detect the entire neuronal population; it was expressed 
      only in 48% of neuron-specific enolase (NSE)-immunoreactive (NSE-IR) neurons. 
      Human neuronal protein appeared to occur in the large majority or all neurons. 
      Almost all CALB-IR neurons were: (1) radially multidendritic; (2) eccentric 
      multidendritic; (3) Dogiel type II. CALB-IR occurred in 20-25% of myenteric and 
      65-75% of submucosal neurons in lamb and mature sheep, with higher values in 
      mature sheep. Nearly all CALB-IR neurons were common choline acetyltransferase 
      (cChAT)-IR, whereas only about 20% of cChAT-IR somata were CALB-IR. In lamb and 
      mature sheep, 90% of MP CALB-IR neurons were peripheral choline acetyltransferase 
      (pChAT)-IR. In lamb SMP, 80+/-13% of CALB-IR cells were also pChAT-IR, whereas 
      all those in mature SMP were pChAT-IR. Fewer myenteric CALB-IR neurons exhibited 
      tachykinin (TK) in mature sheep (49%) than in lamb (88%). This was also the case 
      for submucosal ganglia (mature sheep, 63%; lamb, 89%). In lamb MP, 77+/-7% of 
      CALB-IR cells were NeuN-positive. In mature sheep, 73+/-10% of CALB-IR somata 
      were NeuN-IR, but NeuN failed to stain SMP neurons. In the MP of suckling and 
      mature sheep, Dogiel type II CALB-IR neurons were calcitonin gene-related peptide 
      (CGRP)-IR. In the SMP at both stages, Dogiel type II CALB-IR somata (about 50% of 
      CALB-IR neurons) were also CGRP-IR. Only small proportions of CALB-IR neurons 
      showed immunoreactivity for calretinin or nitric oxide synthase (NOS), although 
      large populations of CALB and NOS neurons occurred in the ganglia. Thus, CALB is 
      a marker of most Dogiel type II neurons in the sheep but is not confined to 
      Dogiel II neurons. CGRP is a more selective marker of Dogiel type II neurons, 
      being only found in this neuron type.
FAU - Chiocchetti, Roberto
AU  - Chiocchetti R
AD  - Department of Veterinary Morphophysiology and Animal Production, University of 
      Bologna, 40064 Ozzano Emilia, Bologna, Italy. chiocchetti@vet.unibo.it
FAU - Grandis, Annamaria
AU  - Grandis A
FAU - Bombardi, Cristiano
AU  - Bombardi C
FAU - Clavenzani, Paolo
AU  - Clavenzani P
FAU - Costerbosa, Giovanna Lalatta
AU  - Costerbosa GL
FAU - Lucchi, Maria L
AU  - Lucchi ML
FAU - Furness, John B
AU  - Furness JB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20040824
PL  - Germany
TA  - Cell Tissue Res
JT  - Cell and tissue research
JID - 0417625
RN  - 0 (Biomarkers)
RN  - 0 (CALB1 protein, human)
RN  - 0 (Calb1 protein, rat)
RN  - 0 (Calbindin 1)
RN  - 0 (Calbindins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (S100 Calcium Binding Protein G)
SB  - IM
MH  - Animals
MH  - Biomarkers/metabolism
MH  - Calbindin 1
MH  - Calbindins
MH  - Enteric Nervous System/*cytology/metabolism
MH  - Female
MH  - Ileum/*innervation
MH  - Lactation/physiology
MH  - Male
MH  - Myenteric Plexus/cytology/metabolism
MH  - Nerve Tissue Proteins/*metabolism
MH  - Neurons/*cytology/metabolism
MH  - S100 Calcium Binding Protein G/*metabolism
MH  - Sheep/*growth & development
MH  - Submucous Plexus/cytology/metabolism
EDAT- 2004/09/01 05:00
MHDA- 2005/03/09 09:00
CRDT- 2004/09/01 05:00
PHST- 2003/10/06 00:00 [received]
PHST- 2004/04/21 00:00 [accepted]
PHST- 2004/09/01 05:00 [pubmed]
PHST- 2005/03/09 09:00 [medline]
PHST- 2004/09/01 05:00 [entrez]
AID - 10.1007/s00441-004-0906-6 [doi]
PST - ppublish
SO  - Cell Tissue Res. 2004 Nov;318(2):289-303. doi: 10.1007/s00441-004-0906-6. Epub 
      2004 Aug 24.