PMID- 15338473
OWN - NLM
STAT- MEDLINE
DCOM- 20050318
LR  - 20131121
IS  - 0263-6484 (Print)
IS  - 0263-6484 (Linking)
VI  - 22
IP  - 5
DP  - 2004 Sep-Oct
TI  - Kupffer cell-derived prostaglandin E2 is involved in regulation of lipid 
      synthesis in rat liver tissue.
PG  - 327-32
AB  - Our recent studies suggest that Kupffer cells play a role in the physiological 
      regulation of lipid metabolism of the adjacent hepatocytes. In the present study, 
      we have tested the hypothesis that inhibition of Kupffer cells decreases 
      prostaglandin E(2) (PGE(2)) release inside liver tissue, a phenomenon 
      contributing to lipid accumulation in hepatocytes. PGE(2) secretion as well as 
      lipid synthesis were assessed in precision-cut liver slices (PCLS) from rats 
      previously treated with Kupffer cell inhibitors (GdCl(3) 10 mg kg(-1) body wt, 
      i.v. injection and glycine 5% in diet). In addition, lipid synthesis was assessed 
      in primary rat hepatocytes cultured in the absence or presence of PGE(2) (0.01, 1 
      and 10 microM). Inhibition of Kupffer cell activity by GdCl(3) decreases PGE(2) 
      secretion by PCLS and resulted in a higher lipid synthesis. Since incubation with 
      PGE(2) over 48 h decreases lipid synthesis from acetate in cultured hepatocytes, 
      we propose that the lower PGE(2) secretion linked to Kupffer cell inhibition, 
      partly explains a higher rate of synthesis of lipids with a subsequent 
      accumulation in liver tissue, as previously shown in fasted rats.
FAU - Neyrinck, Audrey M
AU  - Neyrinck AM
AD  - Unite de Pharmacocinetique, Metabolisme, Nutrition et Toxicologie, Departement 
      des Sciences Pharmaceutiques, Universite Catholique de Louvain, B-1200 Brussels, 
      Belgium.
FAU - Margagliotti, Sabrina
AU  - Margagliotti S
FAU - Gomez, Cristina
AU  - Gomez C
FAU - Delzenne, Nathalie M
AU  - Delzenne NM
LA  - eng
PT  - Journal Article
PL  - England
TA  - Cell Biochem Funct
JT  - Cell biochemistry and function
JID - 8305874
RN  - 0 (Acetates)
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Ketone Bodies)
RN  - 0 (Lipids)
RN  - 0 (Phospholipids)
RN  - 0 (Triglycerides)
RN  - 2V16EO95H1 (Palmitic Acid)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - AU0V1LM3JT (Gadolinium)
RN  - K7Q1JQR04M (Dinoprostone)
RN  - P7082WY76D (gadolinium chloride)
RN  - TE7660XO1C (Glycine)
SB  - IM
MH  - Acetates/metabolism
MH  - Animals
MH  - Anti-Inflammatory Agents/pharmacology
MH  - Cells, Cultured
MH  - Cholesterol/biosynthesis/metabolism
MH  - Dinoprostone/metabolism/pharmacology/*physiology
MH  - Gadolinium/pharmacology
MH  - Glycine/pharmacology
MH  - Hepatocytes/cytology/drug effects/metabolism
MH  - Ketone Bodies/biosynthesis
MH  - Kupffer Cells/drug effects/*metabolism
MH  - Lipids/*biosynthesis
MH  - Liver/drug effects/*metabolism
MH  - Male
MH  - Palmitic Acid/metabolism
MH  - Phospholipids/biosynthesis
MH  - Rats
MH  - Rats, Wistar
MH  - Tissue Culture Techniques
MH  - Triglycerides/biosynthesis
EDAT- 2004/09/01 05:00
MHDA- 2005/03/19 09:00
CRDT- 2004/09/01 05:00
PHST- 2004/09/01 05:00 [pubmed]
PHST- 2005/03/19 09:00 [medline]
PHST- 2004/09/01 05:00 [entrez]
AID - 10.1002/cbf.1110 [doi]
PST - ppublish
SO  - Cell Biochem Funct. 2004 Sep-Oct;22(5):327-32. doi: 10.1002/cbf.1110.