PMID- 1533889
OWN - NLM
STAT- MEDLINE
DCOM- 19920612
LR  - 20071114
IS  - 0023-6837 (Print)
IS  - 0023-6837 (Linking)
VI  - 66
IP  - 4
DP  - 1992 Apr
TI  - Monocyte chemoattractant protein 1 in a rat model of pulmonary granulomatosis.
PG  - 498-503
AB  - Monocyte chemoattractant protein 1 (MCP1), also known as monocyte chemotactic and 
      activating factor, possesses potent chemotactic activity for monocytes and can 
      augment monocyte tumoristatic activity against some tumor cell lines. While these 
      activities suggest a role in inflammatory and immunologic processes, the biologic 
      role of MCP1 has not been studied in vivo. Glucan-induced pulmonary 
      granulomatosis in the rat is an ideal model in which to study the role of MCP1 
      because the granulomas are monocyte/macrophage rich. Intravenous infusion of 
      particulate yeast cell wall glucan resulted in the synchronous development of 
      angiocentric pulmonary granulomas. Early lesions (6 hours) were characterized by 
      intravascular glucan aggregates surrounded by neutrophils and foci of alveolar 
      hemorrhage while later appearing granulomatous lesions (48 to 96 hours) were 
      dominated by monocytes and macrophages. Granuloma formation was paralleled by a 
      peripheral blood monocytosis. Analysis of bronchoalveolar lavage (BAL) fluid 
      revealed an early, transient rise in tumor necrosis factor activity followed by a 
      marked rise in monocyte-specific chemotactic activity. The rise in BAL fluid 
      monocyte chemotactic activity, which coincided with the development of the 
      monocyte/macrophage-rich granulomas, was preceded by a marked increase in whole 
      lung MCP1 mRNA expression. BAL fluid monocyte chemotactic activity could be 
      nearly completely neutralized with antibody directed against rat MCP1. These 
      studies demonstrate that MCP1 mRNA expression is upregulated in glucan-induced 
      pulmonary granulomatosis and that MCP1 is present in BAL fluid. Intrapulmonary 
      granulomatosis may be important in the pathogenesis of granuloma formation.
FAU - Jones, M L
AU  - Jones ML
AD  - Department of Pathology, University of Michigan Medical School, Ann Arbor.
FAU - Warren, J S
AU  - Warren JS
LA  - eng
GR  - HL-40526/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Lab Invest
JT  - Laboratory investigation; a journal of technical methods and pathology
JID - 0376617
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemotactic Factors)
RN  - 0 (Glucans)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Bronchoalveolar Lavage Fluid/chemistry
MH  - Chemokine CCL2
MH  - Chemotactic Factors/*biosynthesis
MH  - Disease Models, Animal
MH  - Glucans/toxicity
MH  - Granuloma/chemically induced/*metabolism
MH  - Lung Diseases/chemically induced/*metabolism
MH  - Male
MH  - Molecular Sequence Data
MH  - RNA, Messenger/*analysis
MH  - Rats
MH  - Specific Pathogen-Free Organisms
MH  - Tumor Necrosis Factor-alpha/biosynthesis
EDAT- 1992/04/01 00:00
MHDA- 1992/04/01 00:01
CRDT- 1992/04/01 00:00
PHST- 1992/04/01 00:00 [pubmed]
PHST- 1992/04/01 00:01 [medline]
PHST- 1992/04/01 00:00 [entrez]
PST - ppublish
SO  - Lab Invest. 1992 Apr;66(4):498-503.