PMID- 15342681
OWN - NLM
STAT- MEDLINE
DCOM- 20050421
LR  - 20210217
IS  - 0022-2275 (Print)
IS  - 0022-2275 (Linking)
VI  - 45
IP  - 11
DP  - 2004 Nov
TI  - Fatty acid binding proteins stabilize leukotriene A4: competition with 
      arachidonic acid but not other lipoxygenase products.
PG  - 2138-44
AB  - Leukotriene A(4) (LTA(4)) is a chemically reactive conjugated triene epoxide 
      product derived from 5-lipoxygenase oxygenation of arachidonic acid. At 
      physiological pH, this reactive compound has a half-life of less than 3 s at 37 
      degrees C and approximately 40 s at 4 degrees C. Regardless of this aqueous 
      instability, LTA(4) is an intermediate in the formation of biologically active 
      leukotrienes, which can be formed through either intracellular or transcellular 
      biosynthesis. Previously, epithelial fatty acid binding protein (E-FABP) present 
      in RBL-1 cells was shown to increase the half-life of LTA(4) to approximately 20 
      min at 4 degrees C. Five FABPs (adipocyte FABP, intestinal FABP, E-FABP, 
      heart/muscle FABP, and liver FABP) have now been examined and also found to 
      increase the half-life of LTA(4) at 4 degrees C to approximately 20 min with 
      protein present. Stabilization of LTA(4) was examined when arachidonic acid was 
      present to compete with LTA(4) for the binding site on E-FABP. Arachidonate has 
      an apparent higher affinity for E-FABP than LTA(4) and was able to completely 
      block stabilization of the latter. When E-FABP is not saturated with 
      arachidonate, FABP can still stabilize LTA(4). Several lipoxygenase products, 
      including 5-hydroxyeicosatetraenoic acid, 5,6-dihydroxyeicosatetraenoic acid, and 
      leukotriene B(4), were found to have no effect on the stability of LTA(4) induced 
      by E-FABP even when present at concentrations 3-fold higher than LTA(4).
FAU - Zimmer, Jennifer S Dickinson
AU  - Zimmer JS
AD  - Department of Pharmacology, University of Colorado Health Sciences Center, 
      Aurora, CO 80045, USA.
FAU - Dyckes, Douglas F
AU  - Dyckes DF
FAU - Bernlohr, David A
AU  - Bernlohr DA
FAU - Murphy, Robert C
AU  - Murphy RC
LA  - eng
GR  - GM-07635/GM/NIGMS NIH HHS/United States
GR  - HL-25785/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20040901
PL  - United States
TA  - J Lipid Res
JT  - Journal of lipid research
JID - 0376606
RN  - 0 (Carrier Proteins)
RN  - 0 (Fatty Acid-Binding Proteins)
RN  - 0 (Hydroxyeicosatetraenoic Acids)
RN  - 0 (Leukotriene A4)
RN  - 27YG812J1I (Arachidonic Acid)
RN  - 467RNW8T91 (5-hydroxy-6,8,11,14-eicosatetraenoic acid)
RN  - 71651-85-9 (5,6-dihydroxy-7,9,11,14-eicosatetraenoic acid)
RN  - EC 1.13.11.12 (Lipoxygenase)
SB  - IM
MH  - Animals
MH  - Arachidonic Acid/*metabolism
MH  - Binding Sites
MH  - Binding, Competitive
MH  - Biochemical Phenomena
MH  - Biochemistry
MH  - Carrier Proteins/*physiology
MH  - Cell Line
MH  - Dose-Response Relationship, Drug
MH  - Fatty Acid-Binding Proteins
MH  - Hydrogen-Ion Concentration
MH  - Hydroxyeicosatetraenoic Acids/pharmacology
MH  - Leukotriene A4/chemistry/*metabolism
MH  - Lipoxygenase/*metabolism
MH  - Mass Spectrometry
MH  - Models, Biological
MH  - Protein Binding
MH  - Rats
MH  - Temperature
MH  - Time Factors
EDAT- 2004/09/03 05:00
MHDA- 2005/04/22 09:00
CRDT- 2004/09/03 05:00
PHST- 2004/09/03 05:00 [pubmed]
PHST- 2005/04/22 09:00 [medline]
PHST- 2004/09/03 05:00 [entrez]
AID - S0022-2275(20)34145-6 [pii]
AID - 10.1194/jlr.M400240-JLR200 [doi]
PST - ppublish
SO  - J Lipid Res. 2004 Nov;45(11):2138-44. doi: 10.1194/jlr.M400240-JLR200. Epub 2004 
      Sep 1.